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GBA (NM_000157) Human Over-expression Lysate

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SKU
LY400055
Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 1 (as WB positive control)
  $436.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216061
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000157, NP_000148
Synonyms GBA1; GCB; GLUC
Predicted MW 59.72 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000148
Locus ID 2629
Cytogenetics 1q22
Protein Families WB
Protein Pathways Lysosome, Metabolic pathways, Other glycan degradation, Sphingolipid metabolism
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Other "GBA" proteins (16)
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PH312242 GBA MS Standard C13 and N15-labeled recombinant protein (NP_001005742) 10 ug
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$3,360.00
LC400055 Lyophilized GBA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423641 Lyophilized GBA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423642 Lyophilized GBA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC425145 Lyophilized GBA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC433165 Lyophilized GBA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY423641 Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 2 (as WB positive control) 100 ug
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LY423642 Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 3 (as WB positive control) 100 ug
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LY425145 Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 3 (as WB positive control) 100 ug
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LY433165 Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 5 (as WB positive control) 100 ug
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TP301614 Purified recombinant protein of Homo sapiens glucosidase, beta; acid (includes glucosylceramidase) (GBA), transcript variant 2, 20 µg 20 ug
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TP312242 Purified recombinant protein of Homo sapiens glucosidase, beta; acid (includes glucosylceramidase) (GBA), transcript variant 3, 20 µg 20 ug
$867.00
TP316061 Recombinant protein of human glucosidase, beta; acid (includes glucosylceramidase) (GBA), transcript variant 1, 20 µg 20 ug
$867.00
TP761812 Purified recombinant protein of Human glucosidase, beta, acid (GBA), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug 50 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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