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CYP1B1 (NM_000104) Human Over-expression Lysate

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SKU
LY400032
Transient overexpression lysate of cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC204074
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000104, NP_000095
Synonyms ASGD6; CP1B; CYPIB1; GLC3A; P4501B1
Predicted MW 60.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000095
Locus ID 1545
Cytogenetics 2p22.2
Protein Families WB
Protein Pathways Metabolism of xenobiotics by cytochrome P450, Tryptophan metabolism
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Other "CYP1B1" proteins (2)
SKU Description Size Price
LC400032 Lyophilized CYP1B1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP762099 Purified recombinant protein of Human cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1),Leu40-Ala305, with N-terminal His tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Cytochrome P450 1B1 inhibition suppresses tumorigenicity of prostate cancer via caspase-1 activation
Chang, Mitsui, Kim et al
Oncotarget (2017) 8 (24), 39087-39100 DOI: 10.18632/oncotarget.16598
FigureFigureFigure
Loss of miR-200c up-regulates CYP1B1 and confers docetaxel resistance in renal cell carcinoma
Chang, Mitsui, Fukuhara et al
Oncotarget (2015) 6 (10), 7774-87
Snippet: For CYP1B1 overexpression, cells were transfected with pCMV6-ENTRY vector expressing human CYP1B1 cDNA or empty pCMV6-ENTRY vector (OriGene Technologies) using Fugene HD Transfection Reagent (Roche Diagnostics) according to the manufacturer's ... Cite SaveSaving
HDAC6 modulates Hsp90 chaperone activity and regulates activation of aryl hydrocarbon receptor signaling
Kekatpure, Dannenberg, Subbaramaiah
J Biol Chem (2009) 284 (12), 7436-45 DOI: 10.1074/jbc.M808999200
AI Summary: OriGene products, specifically the HDAC6 shRNA (SR304274) and human HDAC6 cDNA (SC120389), were used in this study to silence and overexpress HDAC6, respectively, enabling the researchers to investigate the role of HDAC6 in modulating Hsp90 acetylation and aryl hydrocarbon receptor signaling.
Showing 1-3 of 3 papers.
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