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TPM1 (NM_000366) Human Over-expression Lysate

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SKU
LC424765
Lyophilized TPM1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201262
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000366, NP_000357
Synonyms C15orf13; CMD1Y; CMH3; HEL-S-265; HTM-alpha; LVNC9; TMSA
Predicted MW 32.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000357
Locus ID 7168
Cytogenetics 15q22.2
Protein Families WB
Protein Pathways Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)
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Other "TPM1" proteins (23)
SKU Description Size Price
PH301262 TPM1 MS Standard C13 and N15-labeled recombinant protein (NP_000357) 10 ug
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PH318007 TPM1 MS Standard C13 and N15-labeled recombinant protein (NP_001018005) 10 ug
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PH318995 TPM1 MS Standard C13 and N15-labeled recombinant protein (NP_001018020) 10 ug
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PH319543 TPM1 MS Standard C13 and N15-labeled recombinant protein (NP_001018004) 10 ug
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PH319606 TPM1 MS Standard C13 and N15-labeled recombinant protein (NP_001018006) 10 ug
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PH319652 TPM1 MS Standard C13 and N15-labeled recombinant protein (NP_001018007) 10 ug
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LC422687 Lyophilized TPM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422688 Lyophilized TPM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC422689 Lyophilized TPM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422690 Lyophilized TPM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422696 Lyophilized TPM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY422687 Transient overexpression lysate of tropomyosin 1 (alpha) (TPM1), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY422688 Transient overexpression lysate of tropomyosin 1 (alpha) (TPM1), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY422689 Transient overexpression lysate of tropomyosin 1 (alpha) (TPM1), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY422690 Transient overexpression lysate of tropomyosin 1 (alpha) (TPM1), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY422696 Transient overexpression lysate of tropomyosin 1 (alpha) (TPM1), transcript variant 7 (as WB positive control) 100 ug
$436.00
LY424765 Transient overexpression lysate of tropomyosin 1 (alpha) (TPM1), transcript variant 5 (as WB positive control) 100 ug
$436.00
TP301262 Recombinant protein of human tropomyosin 1 (alpha) (TPM1), transcript variant 5, 20 µg 20 ug
$737.00
TP318007 Purified recombinant protein of Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant 1, 20 µg 20 ug
$737.00
TP318995 Purified recombinant protein of Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant 7, 20 µg 20 ug
$737.00
TP319543 Purified recombinant protein of Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant 3, 20 µg 20 ug
$737.00
TP319606 Purified recombinant protein of Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant 4, 20 µg 20 ug
$737.00
TP319652 Purified recombinant protein of Homo sapiens tropomyosin 1 (alpha) (TPM1), transcript variant 2, 20 µg 20 ug
$737.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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