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MMP1 (NM_002421) Human Over-expression Lysate

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SKU
LC419340
Lyophilized MMP1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202460
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002421, NP_002412
Synonyms CLG; CLGN
Predicted MW 54 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002412
Locus ID 4312
Cytogenetics 11q22.2
Protein Families WB
Protein Pathways Bladder cancer, Pathways in cancer, PPAR signaling pathway
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file-alt Citations

Silencing Y-box binding protein-1 inhibits triple-negative breast cancer cell invasiveness via regulation of MMP1 and beta-catenin expression
Lim, Nair, Shyamasundar et al
Cancer Lett. (2019) 452, 119-131 DOI: 10.1016/j.canlet.2019.03.014
AI Summary: OriGene's 29mer shRNA constructs in the retroviral pGFP-V-RS vector were used to specifically knock down YB-1 expression in MDA-MB-231 cells, enabling the study of YB-1's role in triple-negative breast cancer cell invasiveness.
Snippet: 3 x 10 5 parental MDA-MB-231 cells per well were seeded into 6-well plate, and were then transfected with four different 29mer shRNA constructs in retroviral pGFP-V-RS vector (Origene, Rockville, MD, USA) which specifically targets YB-1 protein or scrambled vector
Showing 1-1 of 1 papers.
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