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SGLT2 (SLC5A2) (NM_003041) Human Over-expression Lysate

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SKU
LC418937
Lyophilized SLC5A2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224822
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003041, NP_003032
Synonyms SGLT2
Predicted MW 72.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_003032
Locus ID 6524
Cytogenetics 16p11.2
Protein Families WB
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Other "SGLT2" proteins (1)
SKU Description Size Price
LY418937 Transient overexpression lysate of solute carrier family 5 (sodium/glucose cotransporter), member 2 (SLC5A2) (as WB positive control) 100 ug
$665.00

file-alt Citations

The design and synthesis of novel SGLT2 inhibitors: C-glycosides with benzyltriazolopyridinone and phenylhydantoin as the aglycone moieties
Guo, Hu, DeOrazio et al
Bioorg. Med. Chem. (2014) 22 (13), 3414-22 DOI: 10.1016/j.bmc.2014.04.036
Cited by 43
AI Summary: OriGene Technologies supplied the synthesized cDNAs for human SGLT1 (SLC5A1, NM_000343.1) and SGLT2 (SLC5A2, NM_003041.2), which were essential for generating the cell lines used to evaluate the potency and selectivity of the novel SGLT2 inhibitors described in the study.
Snippet: The cDNAs for the human genes of SGLT1 (SLC5A1—solute carrier family 5 sodium/glucose co-transporter member 1;Accession #NM_000343.1) or SGLT2 (SLC5A2—member 2;Accession #NM_003041.2) were synthesized (OriGene Technologies, Inc.) and sub-cloned into the mammalian expression vector, pcDNA3.1 (Invitrogen Corp., Carlsbad, CA) using standard molecular biology techniques. CHO-K1 cells transfected with pcDNA3.1 (Invitrogen Corp., Carlsbad, CA) containing either the full-length hSGLT1 or hSGLT2 using Lipofectamine 2000 (Invitrogen Corp., Carlsbad, CA) were selected for stable incorporation of the construct by resistance to the selective pressure of Geneticin
Showing 1-1 of 1 papers.
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