INPP4B (NM_003866) Human Over-expression Lysate
SKU
LC418390
Lyophilized INPP4B HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC211134 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_003866, NP_003857 |
| Predicted MW | 104.6 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_003857 |
| Locus ID | 8821 |
| Cytogenetics | 4q31.21 |
| Protein Families | WB |
| Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "INPP4B" proteins (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH311134 | INPP4B MS Standard C13 and N15-labeled recombinant protein (NP_003857) | 10 ug |
$3,360.00
|
|
| PH319612 | INPP4B MS Standard C13 and N15-labeled recombinant protein (NP_001095139) | 10 ug |
$3,360.00
|
|
| LC420324 | Lyophilized INPP4B HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY418390 | Transient overexpression lysate of inositol polyphosphate-4-phosphatase, type II, 105kDa (INPP4B), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| LY420324 | Transient overexpression lysate of inositol polyphosphate-4-phosphatase, type II, 105kDa (INPP4B), transcript variant 2 (as WB positive control) | 100 ug |
$665.00
|
|
| TP311134 | Recombinant protein of human inositol polyphosphate-4-phosphatase, type II, 105kDa (INPP4B), transcript variant 1, 20 µg | 20 ug |
$867.00
|
|
| TP319612 | Recombinant protein of human inositol polyphosphate-4-phosphatase, type II, 105kDa (INPP4B), transcript variant 2, 20 µg | 20 ug |
$867.00
|
|
Citations
INPP4B promotes PI3Kα-dependent late endosome formation and Wnt/β-catenin signaling in breast cancer
Rodgers, Ooms, Oorschot et al
Nat Commun (2021) 12 (1), 3140 DOI: 10.1038/s41467-021-23241-6
Rodgers, Ooms, Oorschot et al
Nat Commun (2021) 12 (1), 3140 DOI: 10.1038/s41467-021-23241-6
Cited by 66
AI Summary: OriGene’s Tissue Scan Breast Cancer cDNA arrays I-IV (BCRT101, BCRT102, BCRT103, and BCRT104) were used to measure INPP4B mRNA expression in primary human breast cancer and normal tissues, contributing essential data for the study's analysis of INPP4B expression patterns.
Snippet: 1b, c). As mRNA expression data were not available for these cohorts, INPP4B mRNA expression was examined using Tissue Scan Breast Cancer cDNA arrays I-IV (OriGene) of 130 primary human breast cancers and 16 normal breast tissues (Fig. 1c);Breast cancer cDNA arrays (176 breast cancers and 16 tumor adjacent normal breast tissues) were purchased from OriGene (BCRT101, BCRT102, BCRT103, and BCRT104)
Epigenetic Inactivation of Inositol polyphosphate 4-phosphatase B (INPP4B), a Regulator of PI3K/AKT Signaling Pathway in EBV-Associated Nasopharyngeal Carcinoma
Yuen, Chung, Lun et al
PLoS ONE (2014) 9 (8), e105163 DOI: 10.1371/journal.pone.0105163
Yuen, Chung, Lun et al
PLoS ONE (2014) 9 (8), e105163 DOI: 10.1371/journal.pone.0105163
Cited by 36
AI Summary: OriGene products, specifically the TrueORF cDNA clone for INPP4B and the pCMV6 expression vector, were used to generate INPP4B expression constructs that enabled the researchers to restore INPP4B expression in nasopharyngeal carcinoma cells and investigate its functional role in tumorigenesis.
Snippet: S1. Restoration of INPP4B expression in C666-1 cells. Full length INPP4B was amplified from a TrueORF cDNA clone (Origene, Rockville, MD) and inserted into pCMV6 (OriGene) to produce the INPP4B expression vector. pCMV6 ... Cite SaveSaving
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
