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SPARCL1 (NM_004684) Human Over-expression Lysate

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SKU
LC417809
Lyophilized SPARCL1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207583
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004684, NP_004675
Synonyms MAST 9; MAST9; PIG33; SC1
Predicted MW 75.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_004675
Locus ID 8404
Cytogenetics 4q22.1
Protein Families WB
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Other "SPARCL1" proteins (7)
SKU Description Size Price
PH307583 SPARCL1 MS Standard C13 and N15-labeled recombinant protein (NP_004675) 10 ug
$3,360.00
PH326078 SPARCL1 MS Standard C13 and N15-labeled recombinant protein (NP_001121782) 10 ug
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LC426947 Lyophilized SPARCL1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY417809 Transient overexpression lysate of SPARC-like 1 (hevin) (SPARCL1), transcript variant 2 (as WB positive control) 100 ug
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LY426947 Transient overexpression lysate of SPARC-like 1 (hevin) (SPARCL1), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP307583 Recombinant protein of human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2, 20 µg 20 ug
$867.00
TP326078 Purified recombinant protein of Homo sapiens SPARC-like 1 (hevin) (SPARCL1), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Methylation of SPARCL1 Is Associated with Oncologic Outcome of Advanced Upper Urinary Tract Urothelial Carcinoma
Luo, Chiang, Huang et al
Int J Mol Sci (2019) 20 (7) DOI: 10.3390/ijms20071653
AI Summary: OriGene contributed to this study by providing the SPARCL1 and GAPDH primers used for gene expression analysis.
Snippet: 3′;SPARCL1 reverse primer: 5′-GTTGGAGGACAAGTCACTGGATC-3′. GAPDH forward: 5′-GTCTCCTCTGACTTCAACAGCG-3′;GAPDH reverse primer: 5′- ACCACCCTGTTGCTGTAGCCAA-3′. All primers were purchased from OriGene (Rockville Cached
FigureFigureFigure
Showing 1-1 of 1 papers.
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