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SPARCL1 (NM_004684) Human Tagged ORF Clone

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SKU
RC207583
SPARCL1 (Myc-DDK-tagged)-Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $618.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPARCL1
Synonyms MAST 9; MAST9; PIG33; SC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207583 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACTGGGCTTTTTTTCCTATGTCTCTTGGGAACTGCAGCTGCAATCCCGACAAATGCAAGATTAT
TATCTGATCATTCCAAACCAACTGCTGAAACGGTAGCACCCGACAACACTGCAATCCCCAGTTTAAGGGC
TGAAGATGAAGAAAATGAAAAAGAAACAGCAGTATCCACAGAAGACGATTCCCACCATAAGGCTGAAAAA
TCATCAGTACTAAAGTCAAAAGAGGAAAGCCATGAACAGTCAGCAGAACAGGGCAAGAGTTCTAGCCAAG
AGCTGGGATTGAAGGATCAAGAGGACAGTGATGGTGACTTAAGTGTGAATTTGGAGTATGCACCAACTGA
AGGTACATTGGACATAAAAGAAGATATGAGTGAGCCTCAGGAGAAAAAACTCTCAGAGAACACTGATTTT
TTGGCTCCTGGTGTTAGTTCCTTCACAGATTCTAACCAACAAGAAAGTATCACAAAGAGAGAGGAAAACC
AAGAACAACCTAGAAATTATTCACATCATCAGTTGAACAGGAGCAGTAAACATAGCCAAGGCCTAAGGGA
TCAAGGAAACCAAGAGCAGGATCCAAATATTTCCAATGGAGAAGAGGAAGAAGAAAAAGAGCCAGGTGAA
GTTGGTACCCACAATGATAACCAAGAAAGAAAGACAGAATTGCCCAGGGAGCATGCTAACAGCAAGCAGG
AGGAAGACAATACCCAATCTGATGATATTTTGGAAGAGTCTGATCAACCAACTCAAGTAAGCAAGATGCA
GGAGGATGAATTTGATCAGGGTAACCAAGAACAAGAAGATAACTCCAATGCAGAAATGGAAGAGGAAAAT
GCATCGAACGTCAATAAGCACATTCAAGAAACTGAATGGCAGAGTCAAGAGGGTAAAACTGGCCTAGAAG
CTATCAGCAACCACAAAGAGACAGAAGAAAAGACTGTTTCTGAGGCTCTGCTCATGGAACCTACTGATGA
TGGTAATACCACGCCCAGAAATCATGGAGTTGATGATGATGGCGATGATGATGGCGATGATGGCGGCACT
GATGGCCCCAGGCACAGTGCAAGTGATGACTACTTCATCCCAAGCCAGGCCTTTCTGGAGGCCGAGAGAG
CTCAATCCATTGCCTATCACCTCAAAATTGAGGAGCAAAGAGAAAAAGTACATGAAAATGAAAATATAGG
TACCACTGAGCCTGGAGAGCACCAAGAGGCCAAGAAAGCAGAGAACTCATCAAATGAGGAGGAAACGTCA
AGTGAAGGCAACATGAGGGTGCATGCTGTGGATTCTTGCATGAGCTTCCAGTGTAAAAGAGGCCACATCT
GTAAGGCAGACCAACAGGGAAAACCTCACTGTGTCTGCCAGGATCCAGTGACTTGTCCTCCAACAAAACC
CCTTGATCAAGTTTGTGGCACTGACAATCAGACCTATGCTAGTTCCTGTCATCTATTCGCTACTAAATGC
AGACTGGAGGGGACCAAAAAGGGGCATCAACTCCAGCTGGATTATTTTGGAGCCTGCAAATCTATTCCTA
CTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACGGATGAGAGACTGGCTCAAGAATATCCTCATGCA
GCTTTATGAAGCCAACTCTGAACACGCTGGTTATCTAAATGAGAAGCAGAGAAATAAAGTCAAGAAAATT
TACCTGGATGAAAAGAGGCTTTTGGCTGGGGACCATCCCATTGACCTTCTCTTAAGGGACTTTAAGAAAA
ACTACCACATGTATGTGTATCCTGTGCACTGGCAGTTTAGTGAACTTGACCAACACCCTATGGATAGAGT
CTTGACACATTCTGAACTTGCTCCTCTGCGAGCATCTCTGGTGCCCATGGAACACTGCATAACCCGTTTC
TTTGAGGAGTGTGACCCCAACAAGGATAAGCACATCACCCTGAAGGAGTGGGGCCACTGCTTTGGAATTA
AAGAAGAGGACATAGATGAAAATCTCTTGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207583 protein sequence
Red=Cloning site Green=Tags(s)

MKTGLFFLCLLGTAAAIPTNARLLSDHSKPTAETVAPDNTAIPSLRAEDEENEKETAVSTEDDSHHKAEK
SSVLKSKEESHEQSAEQGKSSSQELGLKDQEDSDGDLSVNLEYAPTEGTLDIKEDMSEPQEKKLSENTDF
LAPGVSSFTDSNQQESITKREENQEQPRNYSHHQLNRSSKHSQGLRDQGNQEQDPNISNGEEEEEKEPGE
VGTHNDNQERKTELPREHANSKQEEDNTQSDDILEESDQPTQVSKMQEDEFDQGNQEQEDNSNAEMEEEN
ASNVNKHIQETEWQSQEGKTGLEAISNHKETEEKTVSEALLMEPTDDGNTTPRNHGVDDDGDDDGDDGGT
DGPRHSASDDYFIPSQAFLEAERAQSIAYHLKIEEQREKVHENENIGTTEPGEHQEAKKAENSSNEEETS
SEGNMRVHAVDSCMSFQCKRGHICKADQQGKPHCVCQDPVTCPPTKPLDQVCGTDNQTYASSCHLFATKC
RLEGTKKGHQLQLDYFGACKSIPTCTDFEVIQFPLRMRDWLKNILMQLYEANSEHAGYLNEKQRNKVKKI
YLDEKRLLAGDHPIDLLLRDFKKNYHMYVYPVHWQFSELDQHPMDRVLTHSELAPLRASLVPMEHCITRF
FEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004684
ORF Size 1992 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004684.5
RefSeq Size 2910 bp
RefSeq ORF 1995 bp
Locus ID 8404
UniProt ID Q14515, Q8N4S1
Cytogenetics 4q22.1
Domains FOLN, kazal
Protein Families Druggable Genome, Secreted Protein
MW 75.2 kDa
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Documents
Resources
"NM_004684" in other vectors (6)
SKU Description Size Price
RC207583L1 Lenti ORF clone of Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2, Myc-DDK-tagged 10 ug
$918.00
RC207583L2 Lenti ORF clone of Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2, mGFP tagged 10 ug
$918.00
RC207583L3 Lenti ORF clone of Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2, Myc-DDK-tagged 10 ug
$918.00
RC207583L4 Lenti ORF clone of Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2, mGFP tagged 10 ug
$918.00
RG207583 SPARCL1 (tGFP-tagged) - Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2 10 ug
$489.00 $818.00
SC321726 SPARCL1 (untagged)-Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 2 10 ug
$619.00

Plasmid Map for RC207583

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207583
Circular map for RC207583

file-alt Citations

SPARCL1 suppresses metastasis in prostate cancer
Xiang, Qiu, Jiang et al
Mol Oncol (2013)
Cited by 43
AI Summary: OriGene’s human SPARCL1 ORF (RC207583) was used to generate SPARCL1-overexpressing prostate cancer cells, enabling the study to assess SPARCL1’s role in suppressing cancer cell migration, invasiveness, and metastasis.
Snippet: overexpressing SPARCL1. The human gene SPARCL1 ORF (RC207583, OriGene Technologies, Inc, Rockville, MD) was subcloned into pBMN-I-GFP (Addgene Inc., Cambridge, MA, USA) to obtain a pBMN-SPARCL1-I-GFP plasmid.
Showing 1-1 of 1 papers.
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