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SART1 (NM_005146) Human Over-expression Lysate

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SKU
LC417488
Lyophilized SART1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200603
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005146, NP_005137
Synonyms Ara1; HAF; HOMS1; SART1259; SNRNP110; Snu66
Predicted MW 90.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005137
Locus ID 9092
Cytogenetics 11q13.1
Protein Families WB
Protein Pathways Spliceosome
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Documents
Resources
Other "SART1" proteins (1)
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file-alt Citations

Role of the ubiquitin-like protein Hub1 in splice-site usage and alternative splicing
Mishra, Ammon, Popowicz et al
Nature (2011) 474 (7350), 173-8 DOI: 10.1038/nature10143
Cited by 101
AI Summary: In this paper, OriGene’s anti-Hub1 antibody (Cat# TA324319) was used for immunoprecipitation experiments to investigate the interaction between Hub1 and Snu66, thereby contributing to the study of Hub1’s role in splice-site selection and alternative splicing.
Snippet: assays. The antibodies against Hub1 bring down Snu66 from cell extracts and vice versa (Fig. ... domain). GST pull-down assays with purified proteins confirmed that Hub1–Snu66 binding is direct and mediated via HIND (Supplementary Fig.
Showing 1-1 of 1 papers.
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