SART1 (NM_005146) Human Tagged ORF Clone
SKU
RC200603
SART1 (Myc-DDK-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1)
$1,098.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SART1 |
| Synonyms | Ara1; HAF; HOMS1; SART1259; SNRNP110; Snu66 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005146 |
| ORF Size | 2400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005146.5 |
| RefSeq Size | 3601 bp |
| RefSeq ORF | 2403 bp |
| Locus ID | 9092 |
| UniProt ID | O43290 |
| Cytogenetics | 11q13.1 |
| Domains | SART-1 |
| Protein Pathways | Spliceosome |
| MW | 90.7 kDa |
| Summary | This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005146" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200603L1 | Lenti-ORF clone of SART1 (Myc-DDK-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) | 10 ug |
$1,398.00
|
|
| RC200603L2 | Lenti-ORF clone of SART1 (mGFP-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) | 10 ug |
$1,398.00
|
|
| RC200603L3 | Lenti-ORF clone of SART1 (Myc-DDK-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) | 10 ug |
$1,398.00
|
|
| RC200603L4 | Lenti-ORF clone of SART1 (mGFP-tagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) | 10 ug |
$1,398.00
|
|
| RG200603 | SART1 (tGFP-tagged) - Human squamous cell carcinoma antigen recognized by T cells (SART1) | 10 ug |
$1,298.00
|
|
| SC319408 | SART1 (untagged)-Human squamous cell carcinoma antigen recognized by T cells (SART1) | 10 ug |
$1,100.00
|
|
Citations
The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells
Ammon, Mishra, Kowalska et al
J Mol Cell Biol (2014)
Ammon, Mishra, Kowalska et al
J Mol Cell Biol (2014)
Cited by 37
AI Summary: OriGene contributed to this study by providing the cDNA clone for hSnu66 (SART1), which was used to investigate the interaction between Hub1 and spliceosomal proteins in human cells.
Snippet: in pEGFP-N1 (Clontech) or p3x Flag-CMV-10 (Sigma-Aldrich) vectors. The cDNA clone for hSnu66 (SART1) was purchased from Origene, while the cDNA for human Hub1 (UBL5) was amplified by RT–PCR using total RNA from HeLa cells. Plasmids with point mutations ... Related articles All 3 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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