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GRP78 (HSPA5) (NM_005347) Human Over-expression Lysate

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SKU
LC417364
Lyophilized HSPA5 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205859
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005347, NP_005338
Synonyms BIP; GRP78; HEL-S-89n
Predicted MW 72.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005338
Locus ID 3309
Cytogenetics 9q33.3
Protein Families WB
Protein Pathways Antigen processing and presentation, Prion diseases
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Other "GRP78" proteins (4)
SKU Description Size Price
PH305859 HSPA5 MS Standard C13 and N15-labeled recombinant protein (NP_005338) 10 ug
$3,360.00
LY417364 Transient overexpression lysate of heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5) (as WB positive control) 100 ug
$436.00
TP305859 Recombinant protein of human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), 20 µg 20 ug
$867.00
TP701043 Purified recombinant protein of Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), with C-terminal His tag, secretory expressed in HEK293 cells, 50ug 50 ug
$900.00

file-alt Citations

Endoplasmic Reticulum Stress Protein GRP78 Modulates Lipid Metabolism to Control Drug Sensitivity and Antitumor Immunity in Breast Cancer
Cook, Soto-Pantoja, Clarke et al
Cancer Res. (2016) 76 (19), 5657-5670 DOI: 10.1158/0008-5472.CAN-15-2616
AI Summary: OriGene’s GRP78 pcDNA plasmid was used in this study to overexpress GRP78 and investigate its role in regulating lipid metabolism, drug sensitivity, and antitumor immunity in breast cancer cells.
Snippet: GRP78 siRNA was obtained from Dharmacon. GRP78 pcDNA was obtained from Origene
Showing 1-1 of 1 papers.
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