GRP78 (HSPA5) (NM_005347) Human Tagged ORF Clone
SKU
RC205859
HSPA5 (Myc-DDK-tagged)-Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5)
$609.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GRP78 |
| Synonyms | BIP; GRP78; HEL-S-89n |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005347 |
| ORF Size | 1962 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005347.5 |
| RefSeq Size | 3973 bp |
| RefSeq ORF | 1965 bp |
| Locus ID | 3309 |
| UniProt ID | P11021 |
| Cytogenetics | 9q33.3 |
| Domains | HSP70 |
| Protein Families | Druggable Genome |
| Protein Pathways | Antigen processing and presentation, Prion diseases |
| MW | 72.3 kDa |
| Summary | The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. provided by RefSeq, Jul 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005347" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205859L1 | Lenti ORF clone of Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), Myc-DDK-tagged | 10 ug |
$909.00
|
|
| RC205859L2 | Lenti ORF clone of Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), mGFP tagged | 10 ug |
$909.00
|
|
| RC205859L3 | Lenti ORF clone of Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), Myc-DDK-tagged | 10 ug |
$909.00
|
|
| RC205859L4 | Lenti ORF clone of Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), mGFP tagged | 10 ug |
$909.00
|
|
| RG205859 | HSPA5 (tGFP-tagged) - Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5) | 10 ug |
$489.00
$809.00
|
|
| SC108086 | HSPA5 (untagged)-Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5) | 10 ug |
$610.00
|
|
Citations
Nuclear GRP78 Promotes Metabolic Reprogramming and Therapeutic Resistance in Pancreatic Ductal Adenocarcinoma
Zhao, Jiang, Li et al
Clin Cancer Res (2023)
Zhao, Jiang, Li et al
Clin Cancer Res (2023)
α-Mangostin Promotes In Vitro and In Vivo Degradation of Androgen Receptor and AR-V7 Splice Variant in Prostate Cancer Cells
Nauman, Won, Petiwala et al
Cancers (Basel) (2023) 15 (7) DOI: 10.3390/cancers15072118
Nauman, Won, Petiwala et al
Cancers (Basel) (2023) 15 (7) DOI: 10.3390/cancers15072118
Cited by 13
AI Summary: OriGene products contributed to this study by providing the AR (androgen receptor) and AR-V7 expression plasmids (Cat# RC220845 and Cat# RC211706), which were used in transfection experiments to assess the effects of α-mangostin on androgen receptor degradation in prostate cancer cells.
Snippet: 20,21]. Our group has been studying xanthones for their in vitro and in vivo anticancer activity, and our data suggests that xanthones promote apoptosis in PCa cells, degrade AR, and slow prostate tumorigenesis in mice [22,23,24,25,26,27,28,29]. This study was undertaken to test our hypothesis that α-mangostin, the most abundant mangosteen xanthone, promotes the ubiquitination and degradation of
GRP 78 antibodies are associated with clinical phenotype in neuromyelitis optica
Shimizu, Takeshita, Hamamoto et al
Ann Clin Transl Neurol (2019) 6 (10), 2079-2087 DOI: 10.1002/acn3.50905
Shimizu, Takeshita, Hamamoto et al
Ann Clin Transl Neurol (2019) 6 (10), 2079-2087 DOI: 10.1002/acn3.50905
AI Summary: OriGene’s FLAG-tagged GRP78 protein was used in immunoprecipitation experiments to assess the interaction between patient-derived IgG and GRP78, helping to elucidate the association of GRP78 autoantibodies with clinical phenotypes in neuromyelitis optica.
Snippet: For immunoprecipitation, 500 μg/mL of LETM‐IgG (Patient 3) or ON‐IgG (Patient 17) was incubated with 5 μg of HEK 293T cell lysates with or without the overexpression of FLAG‐tagged GRP78 (Origene) for 4 h at 4°C, and then incubated with 40 _μ_L of anti‐FLAG‐IgG coupling resin (EZview Red Anti‐FLAG M2 Affinity Gel beads;Sigma‐Aldrich), for 2 h at 4°C. After the GRP78 anti
GRP78 antibodies damage the blood-brain barrier and relate to cerebellar degeneration in Lambert-Eaton myasthenic syndrome
Shimizu, Takeshita, Sano et al
Brain (2019)
Shimizu, Takeshita, Sano et al
Brain (2019)
AI Summary: OriGene’s FLAG-tagged GRP78 was used to overexpress GRP78 in HEK 293T cell lysates for immunoprecipitation assays, enabling the identification of GRP78 autoantibodies in patient IgG and their role in blood-brain barrier dysfunction in Lambert-Eaton myasthenic syndrome.
Snippet: For immunoprecipitation, 125 or 62.5 μg/ml PCD-LEMS IgG (Patients 3 and 5) or LEMS IgG (Patient 9) was incubated with 5 μg HEK 293T cell lysates with or without the over- expression of FLAG-tagged GRP78 (Origene) for 4h at 4 C, and then incubated with 40 μl
AR-12 suppresses dengue virus replication by down-regulation of PI3K/AKT and GRP78
Chen, Chen, Lin et al
Antiviral Res. (2017)
Chen, Chen, Lin et al
Antiviral Res. (2017)
Integrated cell and process engineering for improved transient production of a "difficult-to-express" fusion protein by CHO cells
Johari, Estes, Alves et al
Biotechnol. Bioeng. (2015)
Johari, Estes, Alves et al
Biotechnol. Bioeng. (2015)
Cited by 78
AI Summary: OriGene products contributed human BiP (GRP78), human protein disulfide isomerase (PDI), and human cyclophilin B (CypB) plasmids, which were used to co-express these ER-resident molecular chaperones in CHO cells to evaluate their effects on improving production and reducing aggregation of the difficult-to-express Sp35Fc fusion protein.
Showing 1-6 of 6 papers.
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