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SAMHD1 (NM_015474) Human Over-expression Lysate

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SKU
LC414526
Lyophilized SAMHD1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206013
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015474, NP_056289
Synonyms CHBL2; DCIP; HDDC1; hSAMHD1; MOP-5; SBBI88
Predicted MW 72.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_056289
Locus ID 25939
Cytogenetics 20q11.23
Protein Families WB
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Other "SAMHD1" proteins (3)
SKU Description Size Price
PH306013 SAMHD1 MS Standard C13 and N15-labeled recombinant protein (NP_056289) 10 ug
$3,360.00
LY414526 Transient overexpression lysate of SAM domain and HD domain 1 (SAMHD1) (as WB positive control) 100 ug
$436.00
TP306013 Recombinant protein of human SAM domain and HD domain 1 (SAMHD1), 20 µg 20 ug
$867.00

file-alt Citations

Interferon γ and α Have Differential Effects on SAMHD1, a Potent Antiviral Protein, in Feline Lymphocytes
Asadian, Bienzle
Viruses (2019) 11 (10) DOI: 10.3390/v11100921
AI Summary: OriGene's SAMHD1 antibody (clone OTI1A1) conjugated to Alexa Fluor 488 was used in this study to detect and analyze SAMHD1 protein expression and localization in feline lymphocytes.
Snippet: The SAMHD1 antibody (clone OTI1A1, OriGene, Rockville, MD, USA) conjugated to Alexa Fluor 488, or the pSAMHD1 antibody (clone T592p, EMD Millipore, Billerica, MA, USA) that conjugated to Pacific Blue (Zenon labeling kit, Molecular Probes, Thermo Fisher, Mississauga Cached
FC
The expression profile of sterile alpha motif and histidine-aspartate domain-containing protein 1 (SAMHD1) in feline tissues
Asadian, Finnie, Bienzle
Vet Immunol Immunopathol (2018) 195, 7-18 DOI: 10.1016/j.vetimm.2017.11.003
Cited by 4
AI Summary: OriGene products contributed to this study by providing the anti-SAMHD1 antibody (Cat# TA326133), which was used for immunohistochemistry to detect SAMHD1 expression in various feline tissues.
The expression profile of sterile alpha motif and histidine-aspartate domain-containing protein 1 (SAMHD1) in feline tissues
Asadian, Finnie, Bienzle
Veterinary Immunology and Immunopathology (2017) 195, 7
AI Summary: OriGene contributed to the study by providing the mouse monoclonal antibody 1A1 for SAMHD1 detection and the HEK293T cell lysate overexpressing SAMHD1 (LY414526) as a positive control.
Snippet: Slides were then incubated overnight with primary antibody (mouse monoclonal antibody 1A1, IgG2b;OriGene, Rockville, MD) diluted 1:100 in wash buffer A lysate of HEK293T cells with transiently overexpressed SAMHD1 served as positive control (LY414526;OriGene
Showing 1-3 of 3 papers.
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