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CLPB (NM_030813) Human Over-expression Lysate

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SKU
LC410684
Lyophilized CLPB HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203013
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_030813, NP_110440
Synonyms ANKCLB; HSP78; MEGCANN; MGCA7; SKD3
Predicted MW 78.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_110440
Locus ID 81570
Cytogenetics 11q13.4
Protein Families WB
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Other "CLPB" proteins (2)
SKU Description Size Price
LY410684 Transient overexpression lysate of ClpB caseinolytic peptidase B homolog (E. coli) (CLPB) (as WB positive control) 100 ug
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TP761474 Purified recombinant protein of Human ClpB caseinolytic peptidase B homolog (E.coli) (CLPB), full length, with N-terminal HIS tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

Human CLPB forms ATP-dependent complexes in the mitochondrial intermembrane space
Thevarajan, Zolkiewski, Zolkiewska
Int. J. Biochem. Cell Biol. (2020) 127, 105841 DOI: 10.1016/j.biocel.2020.105841
Cited by 22
AI Summary: OriGene's pCMV6-Entry vector containing human CLPB with a C-terminal Myc/FLAG tag (Cat# RC203013) was used for transient overexpression of CLPB to study its localization and complex formation in human cells.
Snippet: … Transient overexpression of human CLPB. Cells were transfected with pCMV6-Entry vector containing an insert encoding human CLPB (NM_030813) with a C-terminal Myc/FLAG (OriGene Technologies, Rockville, MD;#RC203013);- 2.3. Transient overexpression of human CLPB. Cells were transfected with pCMV6-Entry vector containing an insert encoding human CLPB (NM_030813) with a C-terminal Myc/FLAG (OriGene Technologies, Rockville, MD
Showing 1-1 of 1 papers.
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