CLPB (NM_030813) Human Tagged ORF Clone

SKU RC203013

CLPB (Myc-DDK-tagged)-Human ClpB caseinolytic peptidase B homolog (E. coli) (CLPB)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CLPB
• Synonyms: ANKCLB; HSP78; MEGCANN; MGCA7; SKD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203013 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTGGGGTCCCTGGTGTTGAGGAGAAAAGCACTGGCGCCACGGCTACTCCTCCGGCTGCTCAGGTCCC; CAACGCTCCGGGGCCATGGAGGTGCTTCCGGCCGGAATGTGACTACTGGGAGTCTCGGGGAGCCGCAGTG; GCTGAGGGTAGCCACCGGGGGGCGCCCTGGAACATCGCCGGCCTTGTTCTCCGGACGTGGGGCAGCCACC; GGGGGGCGCCAGGGAGGACGCTTCGATACCAAATGCCTCGCGGCTGCCACTTGGGGACGCCTTCCTGGTC; CCGAAGAAACACTCCCAGGACAGGACAGCTGGAACGGGGTCCCCAGCAGGGCCGGACTGGGCATGTGCGC; CCTGGCCGCAGCGCTGGTGGTTCATTGCTACAGCAAGAGTCCGTCCAACAAGGATGCAGCCCTGTTGGAA; GCTGCCCGTGCCAACAATATGCAAGAAGTCAGCAGGCTGTTGTCAGAAGGTGCAGATGTCAATGCAAAGC; ACAGACTTGGCTGGACAGCACTCATGGTGGCAGCCATCAACCGAAACAACAGTGTGGTACAGGTCCTGCT; TGCTGCTGGGGCTGATCCAAACCTTGGAGATGATTTCAGCAGTGTTTACAAGACTGCCAAGGAACAGGGA; ATCCATTCTTTGGAAGATGGGGGACAGGACGGTGCAAGCCGGCACATCACAAACCAGTGGACAAGTGCCC; TGGAGTTCAGGAGATGGCTAGGACTCCCCGCTGGCGTCCTGATCACCCGAGAGGATGACTTCAACAACAG; GCTGAACAACCGCGCCAGTTTCAAGGGCTGCACGGCCTTGCACTATGCTGTTCTTGCTGATGACTACCGC; ACTGTCAAGGAGCTGCTTGATGGAGGAGCCAACCCCCTGCAGAGGAATGAAATGGGACACACACCCTTGG; ATTATGCCCGAGAAGGGGAAGTGATGAAGCTTCTGAGGACTTCTGAAGCCAAGTACCAAGAGAAGCAGCG; GAAGCGTGAGGCTGAGGAGCGGCGCCGCTTCCCCCTGGAGCAGCGACTAAAGGAGCACATCATTGGCCAG; GAGAGCGCCATCGCCACAGTGGGTGCTGCGATCCGGAGGAAGGAGAATGGCTGGTACGATGAAGAACACC; CTCTGGTCTTCCTCTTCTTGGGATCATCTGGAATAGGAAAAACAGAGCTGGCCAAGCAGACAGCCAAATA; TATGCACAAAGATGCTAAAAAGGGCTTCATCAGGCTGGACATGTCCGAGTTCCAGGAGCGACACGAGGTG; GCCAAGTTTATTGGGTCTCCACCAGGCTACGTTGGCCATGAGGAGGGTGGCCAGCTGACCAAGAAGTTGA; AGCAGTGCCCCAATGCTGTGGTGCTCTTTGATGAAGTAGACAAGGCCCATCCAGATGTGCTCACCATCAT; GCTGCAGCTGTTTGATGAGGGCCGGCTGACAGATGGAAAAGGGAAGACCATTGATTGCAAGGACGCCATC; TTCATCATGACCTCCAATGTGGCCAGCGACGAGATCGCACAGCACGCGCTGCAGCTGAGGCAGGAAGCTT; TGGAGATGAGCCGTAACCGTATTGCCGAAAACCTGGGGGATGTCCAGATAAGTGACAAGATCACCATCTC; AAAGAACTTCAAGGAGAATGTGATTCGCCCTATCCTGAAAGCTCACTTCCGGAGGGATGAGTTTCTGGGA; CGGATCAATGAGATCGTCTACTTCCTCCCCTTCTGCCACTCGGAGCTCATCCAACTCGTCAACAAGGAAC; TAAACTTCTGGGCCAAGAGAGCCAAGCAAAGGCACAACATCACGCTGCTCTGGGACCGCGAGGTGGCAGA; TGTGCTGGTCGACGGCTACAATGTGCACTATGGCGCCCGCTCCATCAAACATGAGGTAGAACGCCGTGTG; GTGAACCAGCTGGCAGCAGCCTATGAGCAGGACCTGCTGCCAGGGGGCTGTACTTTGCGCATCACGGTGG; AGGACTCAGACAAGCAGCTACTCAAAAGCCCAGAACTGCCCTCACCCCAGGCTGAGAAGCGCCTCCCCAA; GCTGCGTCTGGAGATCATCGACAAGGACAGCAAGACTCGCAGACTGGACATCCGGGCACCACTGCACCCT; GAGAAGGTGTGCAACACCATC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203013 protein sequence; Red=Cloning site Green=Tags(s); ; MLGSLVLRRKALAPRLLLRLLRSPTLRGHGGASGRNVTTGSLGEPQWLRVATGGRPGTSPALFSGRGAAT; GGRQGGRFDTKCLAAATWGRLPGPEETLPGQDSWNGVPSRAGLGMCALAAALVVHCYSKSPSNKDAALLE; AARANNMQEVSRLLSEGADVNAKHRLGWTALMVAAINRNNSVVQVLLAAGADPNLGDDFSSVYKTAKEQG; IHSLEDGGQDGASRHITNQWTSALEFRRWLGLPAGVLITREDDFNNRLNNRASFKGCTALHYAVLADDYR; TVKELLDGGANPLQRNEMGHTPLDYAREGEVMKLLRTSEAKYQEKQRKREAEERRRFPLEQRLKEHIIGQ; ESAIATVGAAIRRKENGWYDEEHPLVFLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEV; AKFIGSPPGYVGHEEGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDGKGKTIDCKDAI; FIMTSNVASDEIAQHALQLRQEALEMSRNRIAENLGDVQISDKITISKNFKENVIRPILKAHFRRDEFLG; RINEIVYFLPFCHSELIQLVNKELNFWAKRAKQRHNITLLWDREVADVLVDGYNVHYGARSIKHEVERRV; VNQLAAAYEQDLLPGGCTLRITVEDSDKQLLKSPELPSPQAEKRLPKLRLEIIDKDSKTRRLDIRAPLHP; EKVCNTI; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030813
• ORF Size: 2121 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030813.6
• RefSeq Size: 3272 bp
• RefSeq ORF: 2124 bp
• Locus ID: 81570
• UniProt ID: Q9H078
• Cytogenetics: 11q13.4
• MW: 78.7 kDa
• Summary: This gene belongs to the ATP-ases associated with diverse cellular activities (AAA+) superfamily. Members of this superfamily form ring-shaped homo-hexamers and have highly conserved ATPase domains that are involved in various processes including DNA replication, protein degradation and reactivation of misfolded proteins. All members of this family hydrolyze ATP through their AAA+ domains and use the energy generated through ATP hydrolysis to exert mechanical force on their substrates. In addition to an AAA+ domain, the protein encoded by this gene contains a C-terminal D2 domain, which is characteristic of the AAA+ subfamily of Caseinolytic peptidases to which this protein belongs. It cooperates with Hsp70 in the disaggregation of protein aggregates. Allelic variants of this gene are associated with 3-methylglutaconic aciduria, which causes cataracts and neutropenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]