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APE1 (APEX1) (NM_080649) Human Over-expression Lysate

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SKU
LC409119
Lyophilized APEX1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213298
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_080649, NP_542380
Synonyms APE; APE1; APEN; APEX; APX; HAP1; REF1
Predicted MW 35.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_542380
Locus ID 328
Cytogenetics 14q11.2
Protein Families WB
Protein Pathways Base excision repair
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Other "APE1" proteins (13)
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LC400618 Lyophilized APEX1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC429949 Lyophilized APEX1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY400618 Transient overexpression lysate of APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1 (as WB positive control) 100 ug
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LY409118 Transient overexpression lysate of APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 2 (as WB positive control) 100 ug
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LY409119 Transient overexpression lysate of APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 3 (as WB positive control) 100 ug
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LY429949 Transient overexpression lysate of APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 3 (as WB positive control) 100 ug
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TP301208 Recombinant protein of human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 2, 20 µg 20 ug
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TP313298 Recombinant protein of human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 3, 20 µg 20 ug
$867.00
TP720082 Recombinant protein of human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1 10 ug
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TP760021 Recombinant protein of human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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