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TBL1Y (NM_134259) Human Over-expression Lysate

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SKU
LC408756
Lyophilized TBL1Y HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212240
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_134259, NP_599021
Synonyms DFNY2; TBL1
Predicted MW 56.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_599021
Locus ID 90665
Cytogenetics Yp11.2
Protein Families WB
Protein Pathways Wnt signaling pathway
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Other "TBL1Y" proteins (11)
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PH312240 TBL1Y MS Standard C13 and N15-labeled recombinant protein (NP_599021) 10 ug
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PH314764 TBL1Y MS Standard C13 and N15-labeled recombinant protein (NP_599020) 10 ug
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LC408755 Lyophilized TBL1Y HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC409606 Lyophilized TBL1Y HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY408755 Transient overexpression lysate of transducin (beta)-like 1, Y-linked (TBL1Y), transcript variant 2 (as WB positive control) 100 ug
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LY408756 Transient overexpression lysate of transducin (beta)-like 1, Y-linked (TBL1Y), transcript variant 3 (as WB positive control) 100 ug
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LY409606 Transient overexpression lysate of transducin (beta)-like 1, Y-linked (TBL1Y), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP312240 Recombinant protein of human transducin (beta)-like 1Y-linked (TBL1Y), transcript variant 3, 20 µg 20 ug
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TP314764 Recombinant protein of human transducin (beta)-like 1Y-linked (TBL1Y), transcript variant 2, 20 µg 20 ug
$867.00
TP320139 Recombinant protein of human transducin (beta)-like 1Y-linked (TBL1Y), transcript variant 1, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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