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GPRC6A (NM_148963) Human Over-expression Lysate

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SKU
LC407708
Lyophilized GPRC6A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218656
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_148963, NP_683766
Synonyms bA86F4.3; GPCR
Predicted MW 104.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_683766
Locus ID 222545
Cytogenetics 6q22.1
Protein Families WB
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Other "GPRC6A" proteins (2)
SKU Description Size Price
LY407708 Transient overexpression lysate of G protein-coupled receptor, family C, group 6, member A (GPRC6A) (as WB positive control) 100 ug
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TP762297 Purified recombinant protein of Human G protein-coupled receptor, family C, group 6, member A (GPRC6A), Gln19-Gln270, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

SHBG141-161 Domain-Peptide Stimulates GPRC6A-Mediated Response in Leydig and β-Langerhans cell lines
De Toni, Guidolin, De Filippis et al
Sci Rep (2019) 9 (1), 19432 DOI: 10.1038/s41598-019-55941-x
Cited by 10
AI Summary: OriGene Technologies contributed to this study by providing the GPRC6A-pcDNA expression vector, which was used to transfect HEK-293T cells for receptor binding and functional assays.
Snippet: … Expression vector containing wild-type (WT) human GPRC6A cDNA (OriGene Technologies, Inc) was used according to manufacturer’s instruction. Briefly, cultured HEK- 293 T cells at 70% of confluence were transfected by FuGENE 6 (Roche Diagnostics) with 1 μg/well of GPRC6A-pcDNA and maintained for 48 hours, followed by overnight starving in serum-free condition to achieve quiescence. …
FigureFigureFigure
Membrane androgen receptor characteristics of human ZIP9 (SLC39A) zinc transporter in prostate cancer cells: Androgen-specific activation and involvement of an inhibitory G protein in zinc and MAP kinase signaling
Thomas, Pang, Dong
Mol. Cell. Endocrinol. (2017)
AI Summary: OriGene products contributed to the paper by providing the human ZIP9 (SLC39A9) cDNA clone and human GPRC6A cDNA clone, which were used to generate transfected cell lines for studying membrane androgen receptor functions in prostate cancer cells.
Snippet: 2.2. ZIP9 and GPRC6A transfection and cell culture. The human ZIP9 cDNA clone (SLC39A9, OriGene, Rockville, MD), and human GPRC6A cDNA clone (OriGene) were sub-cloned into a pCMV6-Neo mammalian expression vector with a Myc-DDK tag (OriGene). ... All 2 versions Cite SaveSaving
WB
Showing 1-2 of 2 papers.
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