GPRC6A (NM_148963) Human Tagged ORF Clone
SKU
RC218656
GPRC6A (Myc-DDK-tagged)-Human G protein-coupled receptor, family C, group 6, member A (GPRC6A)
$848.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GPRC6A |
| Synonyms | bA86F4.3; GPCR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_148963 |
| ORF Size | 2778 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_148963.4 |
| RefSeq Size | 2860 bp |
| RefSeq ORF | 2781 bp |
| Locus ID | 222545 |
| UniProt ID | Q5T6X5 |
| Cytogenetics | 6q22.1 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 104.6 kDa |
| Summary | Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 PubMed 16199532).supplied by OMIM, Nov 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_148963" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC218656L1 | Lenti ORF clone of Human G protein-coupled receptor, family C, group 6, member A (GPRC6A), Myc-DDK-tagged | 10 ug |
$1,148.00
|
|
| RC218656L2 | Lenti ORF clone of Human G protein-coupled receptor, family C, group 6, member A (GPRC6A), mGFP tagged | 10 ug |
$1,148.00
|
|
| RC218656L3 | Lenti ORF clone of Human G protein-coupled receptor, family C, group 6, member A (GPRC6A), Myc-DDK-tagged | 10 ug |
$1,148.00
|
|
| RC218656L4 | Lenti ORF clone of Human G protein-coupled receptor, family C, group 6, member A (GPRC6A), mGFP tagged | 10 ug |
$1,148.00
|
|
| RG218656 | GPRC6A (tGFP-tagged) - Human G protein-coupled receptor, family C, group 6, member A (GPRC6A) | 10 ug |
$789.00
$1,048.00
|
|
| SC306340 | GPRC6A (untagged)-Human G protein-coupled receptor, family C, group 6, member A (GPRC6A) | 10 ug |
$849.00
|
|
Citations
SHBG141-161 Domain-Peptide Stimulates GPRC6A-Mediated Response in Leydig and β-Langerhans cell lines
De Toni, Guidolin, De Filippis et al
Sci Rep (2019) 9 (1), 19432 DOI: 10.1038/s41598-019-55941-x
De Toni, Guidolin, De Filippis et al
Sci Rep (2019) 9 (1), 19432 DOI: 10.1038/s41598-019-55941-x
Cited by 10
AI Summary: OriGene Technologies contributed to this study by providing the GPRC6A-pcDNA expression vector, which was used to transfect HEK-293T cells for receptor binding and functional assays.
Snippet: … Expression vector containing wild-type (WT) human GPRC6A cDNA (OriGene Technologies, Inc) was used according to manufacturer’s instruction. Briefly, cultured HEK- 293 T cells at 70% of confluence were transfected by FuGENE 6 (Roche Diagnostics) with 1 μg/well of GPRC6A-pcDNA and maintained for 48 hours, followed by overnight starving in serum-free condition to achieve quiescence. …
Membrane androgen receptor characteristics of human ZIP9 (SLC39A) zinc transporter in prostate cancer cells: Androgen-specific activation and involvement of an inhibitory G protein in zinc and MAP kinase signaling
Thomas, Pang, Dong
Mol. Cell. Endocrinol. (2017)
Thomas, Pang, Dong
Mol. Cell. Endocrinol. (2017)
Osteocalcin and Sex Hormone Binding Globulin Compete on a Specific Binding Site of GPRC6A
De Toni, Guidolin, De Filippis et al
Endocrinology (2016)
De Toni, Guidolin, De Filippis et al
Endocrinology (2016)
Cited by 51
POLYMORPHISM rs2274911 OF GPRC6A AS A NOVEL RISK FACTOR FOR TESTIS FAILURE
Toni, Nisio, Speltra et al
J. Clin. Endocrinol. Metab. (2016)
Toni, Nisio, Speltra et al
J. Clin. Endocrinol. Metab. (2016)
Cited by 42
Showing 1-4 of 4 papers.
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