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MSI2 (NM_138962) Human Over-expression Lysate

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SKU
LC403371
Lyophilized MSI2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201003
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_138962, NP_620412
Synonyms MSI2H
Predicted MW 35.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_620412
Locus ID 124540
Cytogenetics 17q22
Protein Families WB
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Other "MSI2" proteins (5)
SKU Description Size Price
PH301003 MSI2 MS Standard C13 and N15-labeled recombinant protein (NP_620412) 10 ug
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LC406896 Lyophilized MSI2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY403371 Transient overexpression lysate of musashi homolog 2 (Drosophila) (MSI2), transcript variant 1 (as WB positive control) 100 ug
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LY406896 Transient overexpression lysate of musashi homolog 2 (Drosophila) (MSI2), transcript variant 2 (as WB positive control) 100 ug
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TP301003 Recombinant protein of human musashi homolog 2 (Drosophila) (MSI2), transcript variant 1, 20 µg 20 ug
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file-alt Citations

Musashi expression in β-cells coordinates insulin expression, apoptosis and proliferation in response to endoplasmic reticulum stress in diabetes
Szabat, Kalynyak, Lim et al
Cell Death Dis (2011) 2, e232 DOI: 10.1038/cddis.2011.119
Cited by 61
AI Summary: OriGene contributed to this study by providing RFP- and GFP-bearing shRNA constructs targeting Msi1 and Msi2, as well as human MSI1-GFP and GFP control overexpression plasmids, which were used to manipulate Musashi gene expression in pancreatic β-cells.
Snippet: Materials and Methods. Reagents. Respective RFP- and GFP-bearing shRNA constructs targeted to Msi1 and Msi2 were purchased from OriGene (Rockville, MD, USA). Overexpression constructs for human MSI1-GFP and GFP control plasmids were purchased from OriGene.
FigureFigureFigure
Showing 1-1 of 1 papers.
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