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VR1 (TRPV1) (NM_080704) Human Over-expression Lysate

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SKU
LC403317
Lyophilized TRPV1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217653
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_080704, NP_542435
Synonyms VR1
Predicted MW 95 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_542435
Locus ID 7442
Cytogenetics 17p13.2
Protein Families WB
Protein Pathways Neuroactive ligand-receptor interaction
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Other "VR1" proteins (7)
SKU Description Size Price
LC409038 Lyophilized TRPV1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC409039 Lyophilized TRPV1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC412871 Lyophilized TRPV1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403317 Transient overexpression lysate of transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 1 (as WB positive control) 100 ug
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LY409038 Transient overexpression lysate of transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 4 (as WB positive control) 100 ug
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LY409039 Transient overexpression lysate of transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 3 (as WB positive control) 100 ug
$665.00
LY412871 Transient overexpression lysate of transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 2 (as WB positive control) 100 ug
$665.00

file-alt Citations

Endothelin-1 potentiates TRPV1-mediated vasoconstriction of human adipose arterioles in a protein kinase C-dependent manner
Korishettar, Nishijima, Wang et al
Br J Pharmacol (2021) 178 (3), 709-725 DOI: 10.1111/bph.15324
Cited by 6
AI Summary: In this study, OriGene products—including the TRPV1 antibody (TA322639) and TRPV1 cDNA (RC211598)—were used to detect and confirm TRPV1 mRNA and protein expression in human resistance arteries, supporting the investigation of TRPV1's role in vasomotor function.
Figure
Intracellular Long-Chain Acyl CoAs Activate TRPV1 Channels
Yu, Carter, Youssef et al
PLoS ONE (2014) 9 (5), e96597 DOI: 10.1371/journal.pone.0096597
Cited by 20
AI Summary: OriGene contributed to this study by providing the full-length human TRPV1 DNA construct, which was used for site-directed mutagenesis experiments to investigate TRPV1 channel regulation.
Snippet: and Felix ratio-metric software (Photon Technologies International, NJ, USA). Site-directed Mutagenesis. The full-length human TRPV1 DNA construct was purchased from OriGene (OriGene Technologies, Rockville, MD, US. Cite SaveSaving
FigureFigureFigure
Showing 1-2 of 2 papers.
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