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PRMT8 (NM_019854) Human Over-expression Lysate

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SKU
LC402741
Lyophilized PRMT8 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205188
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_019854, NP_062828
Synonyms HRMT1L3; HRMT1L4
Predicted MW 45.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_062828
Locus ID 56341
Cytogenetics 12p13.32
Protein Families WB
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Other "PRMT8" proteins (3)
SKU Description Size Price
PH305188 PRMT8 MS Standard C13 and N15-labeled recombinant protein (NP_062828) 10 ug
$3,360.00
LY402741 Transient overexpression lysate of protein arginine methyltransferase 8 (PRMT8) (as WB positive control) 100 ug
$436.00
TP305188 Recombinant protein of human protein arginine methyltransferase 8 (PRMT8), 20 µg 20 ug
$867.00

file-alt Citations

Protein arginine methyltransferase 8 gene enhances the colon cancer stem cell (CSC) function by upregulating the pluripotency transcription factor
Lin, Wang, Yu et al
J Cancer (2018) 9 (8), 1394-1402 DOI: 10.7150/jca.23835
AI Summary: OriGene products, specifically the PRMT8-pMCV plasmid and control vector-pMCV, were used to transfect colon cancer cells, enabling the study of PRMT8's role in enhancing cancer stem cell characteristics by upregulating pluripotency transcription factors.
Snippet: When cells confluence reached 70%-80%, transfection was conducted using LipofectamineTM2000 (Invitrogen). HCT-8 and RKO cells were transfected with PRMT8-pMCV (Origene, USA) or control vector-pMCV (Origene, USA)
FigureFigureFigure
Showing 1-1 of 1 papers.
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