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Plunc (BPIFA1) (NM_016583) Human Over-expression Lysate

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SKU
LC402569
Lyophilized BPIFA1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213322
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_016583, NP_057667
Synonyms bA49G10.5; LUNX; NASG; PLUNC; SPLUNC1; SPURT
Predicted MW 26.71 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_057667
Locus ID 51297
Cytogenetics 20q11.21
Protein Families WB
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Other "Plunc" proteins (7)
SKU Description Size Price
PH303060 PLUNC MS Standard C13 and N15-labeled recombinant protein (NP_570913) 10 ug
$3,360.00
PH313322 PLUNC MS Standard C13 and N15-labeled recombinant protein (NP_057667) 10 ug
$3,360.00
LC408781 Lyophilized BPIFA1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY402569 Transient overexpression lysate of palate, lung and nasal epithelium associated (PLUNC), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY408781 Transient overexpression lysate of palate, lung and nasal epithelium associated (PLUNC), transcript variant 2 (as WB positive control) 100 ug
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TP303060 Recombinant protein of human palate, lung and nasal epithelium associated (PLUNC), transcript variant 2, 20 µg 20 ug
$737.00
TP313322 Recombinant protein of human palate, lung and nasal epithelium associated (PLUNC), transcript variant 1, 20 µg 20 ug
$737.00

file-alt Citations

Immunomodulatory function of the cystic fibrosis modifier gene BPIFA1
Saferali, Tang, Strug et al
PLoS ONE (2020) 15 (1), e0227067 DOI: 10.1371/journal.pone.0227067
Cited by 15
AI Summary: OriGene contributed to this study by providing a human cDNA clone of the complete open reading frame of BPIFA1 (transcript variant 1, RefSeq NM_016583.2), which was used to investigate the immunomodulatory function of BPIFA1 in cystic fibrosis airway epithelial cells.
Snippet: … A human cDNA clone of the complete open reading frame of transcript variant 1 of BPIFA1 (RefSeq NM_016583.2) was purchased from Origene (Origene, Rockville, MD, USA) Cached;;- IB3-1 cells were seeded in 12-well plates and allowed to achieve ~80% density over 1 to 3 days. A human cDNA clone of the complete open reading frame of transcript variant 1 of BPIFA1 (RefSeq NM_016583.2) was purchased from Origene (Origene, Rockville, MD Cached
FigureFigureFigure
Showing 1-1 of 1 papers.
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