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TRPC5 (NM_012471) Human Over-expression Lysate

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SKU
LC402222
Lyophilized TRPC5 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213238
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_012471, NP_036603
Synonyms PPP1R159; TRP5
Predicted MW 111.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_036603
Locus ID 7224
Cytogenetics Xq23
Protein Families WB
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Other "TRPC5" proteins (2)
SKU Description Size Price
LY402222 Transient overexpression lysate of transient receptor potential cation channel, subfamily C, member 5 (TRPC5) (as WB positive control) 100 ug
$665.00
TP762243 Purified recombinant protein of Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5), Pro812-His927, with N-terminal His-ABP tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Englerin A Agonizes the TRPC4/C5 Cation Channels to Inhibit Tumor Cell Line Proliferation
Carson, Raman, Tullai et al
PLoS ONE (2015) 10 (6), e0127498 DOI: 10.1371/journal.pone.0127498
Cited by 109
AI Summary: In this study, the human TRPC4 cDNA obtained from OriGene (catalog number RC218941) was used to construct HEK293T cell lines with inducible TRPC4 expression, enabling the researchers to investigate the role of TRPC4 in mediating the anti-proliferative effects of Englerin A on tumor cells.
Snippet: The HEK293T cell line with Dox-inducible expression of TRPC4 was constructed using the Invitrogen pcDNA5/FRT/TO TOPO TA expression kit (K6020-20). Human TRPC4 was ordered from Origene (Origene;RC218941). Forward ... Cite SaveSaving
FigureFigureFigure
Phospholipase C not protein kinase C is required for the activation of TRPC5 channels by cholecystokinin
Grisanti, Kurada, Cilz et al
Eur. J. Pharmacol. (2012) 689 (1-3), 17-24 DOI: 10.1016/j.ejphar.2012.05.032
Cited by 9
AI Summary: OriGene contributed to this study by providing the TRPC5 channel cDNA construct (subcloned into pCMV6-XL4), which was used for co-expression experiments in HEK293 cells to investigate TRPC5 activation by cholecystokinin.
Snippet: and Xiao et al. 2009a). Briefly, a cDNA construct containing TRPC5 channels (GenBank accession number NM_012471) subcloned into pCMV6-XL4 was purchased from OriGene (Rockville, MD). A cDNA construct containing
Showing 1-2 of 2 papers.
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