DKK1 (NM_012242) Human Over-expression Lysate
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC200094 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_012242, NP_036374 |
| Synonyms | DKK-1; SK |
| Predicted MW | 28.7 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_036374 |
| Locus ID | 22943 |
| Cytogenetics | 10q21.1 |
| Protein Families | WB |
| Protein Pathways | Wnt signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "DKK1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LY402176 | Transient overexpression lysate of dickkopf homolog 1 (Xenopus laevis) (DKK1) (as WB positive control) | 100 ug |
$436.00
|
|
| TP710289 | Purified recombinant protein of Human dickkopf homolog 1 (Xenopus laevis) (DKK1), full length, with C-terminal DDK tag, expressed in sf9, 20ug | 20 ug |
$540.00
|
|
| TP762060 | Purified recombinant protein of Human dickkopf homolog 1 (Xenopus laevis) (DKK1),Thr32-End, with N-terminal His tag, expressed in E. coli, 50ug | 50 ug |
$270.00
|
|
Citations
Combined activity of temozolomide and the mTOR inhibitor temsirolimus in metastatic melanoma involves DKK1
Niessner, Kosnopfel, Sinnberg et al
Exp. Dermatol. (2017)
Niessner, Kosnopfel, Sinnberg et al
Exp. Dermatol. (2017)
AI Summary: In this study, OriGene's anti-DKK1 antibody was used to detect DKK1 protein levels by Western blot, helping to demonstrate that upregulation of DKK1 is associated with increased apoptosis in melanoma cells treated with temozolomide and temsirolimus.
Snippet: Total cell lysates were generated and used in Western Blot analysis as described previously (1). Blots were probed with antibodies directed against DKK1 (Origene, Rockville, MD, USA), Mcl-1, PARP, cleaved PARP, PUMA, phosphorylated S6 ribosomal protein, total ... All 2 versions Cite SaveSaving
Genistein affects histone modifications on Dickkopf-related protein 1 (DKK1) gene in SW480 human colon cancer cell line
Wang, Li, Chen
PLoS ONE (2012) 7 (7), e40955 DOI: 10.1371/journal.pone.0040955
Wang, Li, Chen
PLoS ONE (2012) 7 (7), e40955 DOI: 10.1371/journal.pone.0040955
Cited by 77
AI Summary: OriGene’s DKK1-containing pCMV vector was used to overexpress DKK1 in SW480 colon cancer cells, enabling the study to confirm DKK1's role in genistein-induced growth inhibition.
Snippet: DKK1 Overexpression. SW480 cells were cultured as described above and transfected with either the control pCMV vector or DKK1-containing pCMV (OriGene, Rockville, MD). The empty pCMV vector was used as a transfection control.
Breast cancer-derived Dickkopf1 inhibits osteoblast differentiation and osteoprotegerin expression: implication for breast cancer osteolytic bone metastases
Bu, Lu, Liu et al
Int J Cancer (2008) 123 (5), 1034-42 DOI: 10.1002/ijc.23625
Bu, Lu, Liu et al
Int J Cancer (2008) 123 (5), 1034-42 DOI: 10.1002/ijc.23625
Cited by 126
AI Summary: OriGene’s Human Dkk1 shRNA plasmid (OriGene, Cat# TL313293) was used in this study to silence Dkk1 expression in breast cancer cells, enabling the authors to demonstrate the specific role of Dkk1 in inhibiting osteoblast differentiation and osteoprotegerin expression.
Showing 1-3 of 3 papers.
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