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SF2 (SRSF1) (NM_006924) Human Over-expression Lysate

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SKU
LC402060
Lyophilized SRSF1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Other products for "SF2 or NM_006924"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201636
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006924, NP_008855
Synonyms ASF; SF2; SF2p33; SFRS1; SRp30a
Predicted MW 27.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_008855
Locus ID 6426
Cytogenetics 17q22
Protein Families WB
Protein Pathways Spliceosome
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Other "SF2" proteins (8)
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LC421492 Lyophilized SRSF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY402060 Transient overexpression lysate of splicing factor, arginine/serine-rich 1 (SFRS1), transcript variant 1 (as WB positive control) 100 ug
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LY421492 Transient overexpression lysate of splicing factor, arginine/serine-rich 1 (SFRS1), transcript variant 2 (as WB positive control) 100 ug
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LY425877 Transient overexpression lysate of splicing factor, arginine/serine-rich 1 (SFRS1), transcript variant 2 (as WB positive control) 100 ug
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TP301636 Recombinant protein of human splicing factor, arginine/serine-rich 1 (SFRS1), transcript variant 1, 20 µg 20 ug
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TP761870 Purified recombinant protein of Human serine/arginine-rich splicing factor 1 (SRSF1), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug 50 ug
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file-alt Citations

Direct detection of alternative open reading frames translation products in human significantly expands the proteome
Vanderperre, Lucier, Bissonnette et al
PLoS ONE (2013) 8 (8), e70698 DOI: 10.1371/journal.pone.0070698
Cited by 223
AI Summary: OriGene’s cDNA plasmid clone SC119794 was used to PCR amplify BDRKB2 in this study, enabling the investigation of alternative open reading frame translation products and their contribution to the expanded human proteome.
Snippet: 1A). Reference proteins populate current protein databases used to support research in life sciences. ... PCR fragments were inserted into the BamHI site of pcDNA HA-EGFP+2 . BDRKB2 was PCR amplified from a cDNA containing plasmid (clone SC119794, OriGene), and the
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Showing 1-1 of 1 papers.
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