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PSMD4 (NM_002810) Human Over-expression Lysate

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SKU
LC400997
Lyophilized PSMD4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210046
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002810, NP_002801
Synonyms AF; AF-1; ASF; MCB1; pUB-R5; Rpn10; S5A
Predicted MW 40.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002801
Locus ID 5710
Cytogenetics 1q21.3
Protein Families WB
Protein Pathways Proteasome
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Other "PSMD4" proteins (4)
SKU Description Size Price
PH310046 PSMD4 MS Standard C13 and N15-labeled recombinant protein (NP_002801) 10 ug
$3,360.00
LY400997 Transient overexpression lysate of proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (PSMD4) (as WB positive control) 100 ug
$436.00
TP310046 Recombinant protein of human proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (PSMD4), 20 µg 20 ug
$737.00
TP760263 Recombinant protein of human proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (PSMD4), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Cytoplasmic localization of Nrf2 promotes colorectal cancer with more aggressive tumors via upregulation of PSMD4
Lin, Chang, Wu et al
Free Radic. Biol. Med. (2016)
Cited by 39
AI Summary: In this study, OriGene's PSMD4 overexpression plasmids were used to investigate the role of PSMD4 in promoting colorectal cancer aggressiveness associated with cytoplasmic localization of Nrf2.
Snippet: University, Taichung, Taiwan). β-catenin and CRM1 overexpression plasmids were provided from Addgene (Cambridge, MA). PSMD4 overexpression plasmids were provided from Origene (Rockville, MD). shRNA was purchased ... Cite SaveSaving
Showing 1-1 of 1 papers.
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