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Integrin beta 1 (ITGB1) (NM_002211) Human Over-expression Lysate

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SKU
LC400805
Lyophilized ITGB1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203818
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002211, NP_002202
Synonyms CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB
Predicted MW 88.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002202
Locus ID 3688
Cytogenetics 10p11.22
Protein Families WB
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC), Axon guidance, Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer
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Other "Integrin beta 1" proteins (9)
SKU Description Size Price
PH303818 ITGB1 MS Standard C13 and N15-labeled recombinant protein (NP_002202) 10 ug
$3,360.00
PH315247 ITGB1 MS Standard C13 and N15-labeled recombinant protein (NP_596867) 10 ug
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LC408839 Lyophilized ITGB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC409466 Lyophilized ITGB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400805 Transient overexpression lysate of integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A (as WB positive control) 100 ug
$436.00
LY408839 Transient overexpression lysate of integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1E (as WB positive control) 100 ug
$665.00
LY409466 Transient overexpression lysate of integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1D (as WB positive control) 100 ug
$665.00
TP303818 Recombinant protein of human integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A, 20 µg 20 ug
$867.00
TP315247 Recombinant protein of human integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1E, 20 µg 20 ug
$867.00

file-alt Citations

Twist induces epithelial-mesenchymal transition and cell motility in breast cancer via ITGB1-FAK/ILK signaling axis and its associated downstream network
Yang, Hou, Zhou et al
Int. J. Biochem. Cell Biol. (2015) 71, 62-71 DOI: 10.1016/j.biocel.2015.12.004
Cited by 92
AI Summary: OriGene’s human ITGB1 (NM_002211) expression plasmid was used to overexpress ITGB1 in breast epithelial cells, enabling the study of its role in Twist-induced epithelial-mesenchymal transition and associated signaling pathways.
Snippet: legends. The retroviral expression vectors encoding c-Myc-tagged Twist (Liu et al., 2009) and the human ITGB1 (NM_002211) purchased from Origene (http://www.origene.com) were subcloned into pBABE-Puro-based vector. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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