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FOXC1 (NM_001453) Human Over-expression Lysate

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SKU
LC400565
Lyophilized FOXC1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215629
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001453, NP_001444
Synonyms ARA; ASGD3; FKHL7; FREAC-3; FREAC3; IGDA; IHG1; IRID1; RIEG3
Predicted MW 56.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001444
Locus ID 2296
Cytogenetics 6p25.3
Protein Families WB
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Other "FOXC1" proteins (3)
SKU Description Size Price
LY400565 Transient overexpression lysate of forkhead box C1 (FOXC1) (as WB positive control) 100 ug
$665.00
TP761578 Purified recombinant protein of Human forkhead box C1 (FOXC1), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00
TP762353 Purified recombinant protein of Human forkhead box C1 (FOXC1), Pro197-Gln306, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

FOXC1 plays a crucial role in the growth of pancreatic cancer
Subramani, Camacho, Levin et al
Oncogenesis (2018) 7 (7), 52 DOI: 10.1038/s41389-018-0061-7
AI Summary: OriGene products contributed to this study by providing the FOXC1 cDNA clone for overexpression and FOXC1-specific shRNA constructs for gene silencing, enabling the authors to investigate the functional role of FOXC1 in pancreatic cancer growth and metastasis.
FigureFigureFigure
Showing 1-1 of 1 papers.
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