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ACOT1 (NM_001037161) Human Over-expression Lysate

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SKU
LC400415
Lyophilized ACOT1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221302
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001037161, NP_001032238
Synonyms ACH2; CTE-1; LACH2
Predicted MW 46.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001032238
Locus ID 641371
Cytogenetics 14q24.3
Protein Families WB
Protein Pathways Biosynthesis of unsaturated fatty acids
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Other "ACOT1" proteins (4)
SKU Description Size Price
PH321302 ACOT1 MS Standard C13 and N15-labeled recombinant protein (NP_001032238) 10 ug
$3,360.00
LY400415 Transient overexpression lysate of acyl-CoA thioesterase 1 (ACOT1) (as WB positive control) 100 ug
$665.00
TP321302 Recombinant protein of human acyl-CoA thioesterase 1 (ACOT1), 20 µg 20 ug
$737.00
TP760829 Purified recombinant protein of Human acyl-CoA thioesterase 1 (ACOT1), full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Cardiomyocyte specific expression of Acyl-coA thioesterase 1 attenuates sepsis induced cardiac dysfunction and mortality
Xia, Dong, Chen et al
Biochem. Biophys. Res. Commun. (2015)
Cited by 21
AI Summary: OriGene’s pCMV-ACOT1 plasmid was used to transfect cells and overexpress ACOT1, enabling the authors to study its protective effects against sepsis-induced cardiac dysfunction.
Snippet: Type Tissue Collection (Manassas, Virginia), and were grown in DMEM containing 10% fetal bovine serum in an atmosphere of 95% air and 5% CO 2 at 37 °C. Cells were plated in 6-well plates at a proper density and were transfected with pCMV-ACOT1 (Origene) ... Cite SaveSaving
Showing 1-1 of 1 papers.
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