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IFNAR1 (NM_000629) Human Over-expression Lysate

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SKU
LC400212
Lyophilized IFNAR1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205141
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000629, NP_000620
Synonyms AVP; IFN-alpha-REC; IFNAR; IFNBR; IFRC
Predicted MW 63.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000620
Locus ID 3454
Cytogenetics 21q22.11
Protein Families WB
Protein Pathways Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Toll-like receptor signaling pathway
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Other "IFNAR1" proteins (3)
SKU Description Size Price
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TP762081 Purified recombinant protein of Human interferon (alpha, beta and omega) receptor 1 (IFNAR1),Lys276-Asn416, with N-terminal His tag, expressed in E. coli, 50ug 50 ug
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file-alt Citations

Type-1 interferons contribute to oxygen glucose deprivation induced neuro-inflammation in BE(2)M17 human neuroblastoma cells
Minter, Zhang, Ates et al
J Neuroinflammation (2014) 11 (1), 43 DOI: 10.1186/1742-2094-11-43
Cited by 17
AI Summary: OriGene’s shRNA plasmid constructs targeting IFNAR1 and a non-effective scrambled shRNA control were used to generate knockdown and control BE(2)M17 cell lines, enabling the study to demonstrate the role of type-1 interferon signaling in neuro-inflammation following oxygen glucose deprivation.
Snippet: IFNAR1(IFNAR1KD)ornegativecontrol(NC)knock- downM17cellsweregeneratedusingcommercially availableshRNAplasmidconstructs(Origene).Briefly, M17cellsweretransfectedusingFugene<00AE>HD(Promega) withshRNAplasmidscontaininganIFNAR1specific shRNAcassetteornon-effective29-merscrambledshRNA cassettewithaGFPtag.Clonalcelllinesweregenerated
Showing 1-1 of 1 papers.
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