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IFNAR1 (NM_000629) Human Tagged ORF Clone

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SKU
RC205141
IFNAR1 (Myc-DDK-tagged)-Human interferon (alpha, beta and omega) receptor 1 (IFNAR1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $519.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IFNAR1
Synonyms AVP; IFN-alpha-REC; IFNAR; IFNBR; IFRC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205141 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGTCGTCCTCCTGGGCGCGACGACCCTAGTGCTCGTCGCCGTGGCGCCATGGGTGTTGTCCGCAG
CCGCAGGTGGAAAAAATCTAAAATCTCCTCAAAAAGTAGAGGTCGACATCATAGATGACAACTTTATCCT
GAGGTGGAACAGGAGCGATGAGTCTGTCGGGAATGTGACTTTTTCATTCGATTATCAAAAAACTGGGATG
GATAATTGGATAAAATTGTCTGGGTGTCAGAATATTACTAGTACCAAATGCAACTTTTCTTCACTCAAGC
TGAATGTTTATGAAGAAATTAAATTGCGTATAAGAGCAGAAAAAGAAAACACTTCTTCATGGTATGAGGT
TGACTCATTTACACCATTTCGCAAAGCTCAGATTGGTCCTCCAGAAGTACATTTAGAAGCTGAAGATAAG
GCAATAGTGATACACATCTCTCCTGGAACAAAAGATAGTGTTATGTGGGCTTTGGATGGTTTAAGCTTTA
CATATAGCTTAGTTATCTGGAAAAACTCTTCAGGTGTAGAAGAAAGGATTGAAAATATTTATTCCAGACA
TAAAATTTATAAACTCTCACCAGAGACTACTTATTGTCTAAAAGTTAAAGCAGCACTACTTACGTCATGG
AAAATTGGTGTCTATAGTCCAGTACATTGTATAAAGACCACAGTTGAAAATGAACTACCTCCACCAGAAA
ATATAGAAGTCAGTGTCCAAAATCAGAACTATGTTCTTAAATGGGATTATACATATGCAAACATGACCTT
TCAAGTTCAGTGGCTCCACGCCTTTTTAAAAAGGAATCCTGGAAACCATTTGTATAAATGGAAACAAATA
CCTGACTGTGAAAATGTCAAAACTACCCAGTGTGTCTTTCCTCAAAACGTTTTCCAAAAAGGAATTTACC
TTCTCCGCGTACAAGCATCTGATGGAAATAACACATCTTTTTGGTCTGAAGAGATAAAGTTTGATACTGA
AATACAAGCTTTCCTACTTCCTCCAGTCTTTAACATTAGATCCCTTAGTGATTCATTCCATATCTATATC
GGTGCTCCAAAACAGTCTGGAAACACGCCTGTGATCCAGGATTATCCACTGATTTATGAAATTATTTTTT
GGGAAAACACTTCAAATGCTGAGAGAAAAATTATCGAGAAAAAAACTGATGTTACAGTTCCTAATTTGAA
ACCACTGACTGTATATTGTGTGAAAGCCAGAGCACACACCATGGATGAAAAGCTGAATAAAAGCAGTGTT
TTTAGTGACGCTGTATGTGAGAAAACAAAACCAGGAAATACCTCTAAAATTTGGCTTATAGTTGGAATTT
GTATTGCATTATTTGCTCTCCCGTTTGTCATTTATGCTGCGAAAGTCTTCTTGAGATGCATCAATTATGT
CTTCTTTCCATCACTTAAACCTTCTTCCAGTATAGATGAGTATTTCTCTGAACAGCCATTGAAGAATCTT
CTGCTTTCAACTTCTGAGGAACAAATCGAAAAATGTTTCATAATTGAAAATATAAGCACAATTGCTACAG
TAGAAGAAACTAATCAAACTGATGAAGATCATAAAAAATACAGTTCCCAAACTAGCCAAGATTCAGGAAA
TTATTCTAATGAAGATGAAAGCGAAAGTAAAACAAGTGAAGAACTACAGCAGGACTTTGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205141 protein sequence
Red=Cloning site Green=Tags(s)

MMVVLLGATTLVLVAVAPWVLSAAAGGKNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGM
DNWIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPPEVHLEAEDK
AIVIHISPGTKDSVMWALDGLSFTYSLVIWKNSSGVEERIENIYSRHKIYKLSPETTYCLKVKAALLTSW
KIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWDYTYANMTFQVQWLHAFLKRNPGNHLYKWKQI
PDCENVKTTQCVFPQNVFQKGIYLLRVQASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYI
GAPKQSGNTPVIQDYPLIYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSV
FSDAVCEKTKPGNTSKIWLIVGICIALFALPFVIYAAKVFLRCINYVFFPSLKPSSSIDEYFSEQPLKNL
LLSTSEEQIEKCFIIENISTIATVEETNQTDEDHKKYSSQTSQDSGNYSNEDESESKTSEELQQDFV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000629
ORF Size 1671 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000629.3
RefSeq Size 6099 bp
RefSeq ORF 1674 bp
Locus ID 3454
UniProt ID P17181
Cytogenetics 21q22.11
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Toll-like receptor signaling pathway
MW 63.5 kDa
Summary The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The protein belongs to the type II cytokine receptor family and functions as an antiviral factor. provided by RefSeq, Jul 2020
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Documents
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"NM_000629" in other vectors (7)
SKU Description Size Price
RC205141L1 Lenti ORF clone of Human interferon (alpha, beta and omega) receptor 1 (IFNAR1), Myc-DDK-tagged 10 ug
$819.00
RC205141L2 Lenti ORF clone of Human interferon (alpha, beta and omega) receptor 1 (IFNAR1), mGFP tagged 10 ug
$819.00
RC205141L3 Lenti ORF clone of Human interferon (alpha, beta and omega) receptor 1 (IFNAR1), Myc-DDK-tagged 10 ug
$819.00
RC205141L4 Lenti ORF clone of Human interferon (alpha, beta and omega) receptor 1 (IFNAR1), mGFP tagged 10 ug
$819.00
RG205141 IFNAR1 (tGFP-tagged) - Human interferon (alpha, beta and omega) receptor 1 (IFNAR1) 10 ug
$489.00 $719.00
SC119799 IFNAR1 (untagged)-Human interferon (alpha, beta and omega) receptor 1 (IFNAR1) 10 ug
$519.00
SC324178 IFNAR1 (untagged)-Human interferon (alpha, beta and omega) receptor 1 (IFNAR1) 10 ug
$519.00

Plasmid Map for RC205141

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205141
Circular map for RC205141

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