CYP1A1 (NM_000499) Human Over-expression Lysate
SKU
LC400170
Lyophilized CYP1A1 HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC205760 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_000499, NP_000490 |
| Synonyms | AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX |
| Predicted MW | 58.2 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_000490 |
| Locus ID | 1543 |
| Cytogenetics | 15q24.1 |
| Protein Families | WB |
| Protein Pathways | Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tryptophan metabolism |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CYP1A1" proteins (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH305760 | CYP1A1 MS Standard C13 and N15-labeled recombinant protein (NP_000490) | 10 ug |
$3,360.00
|
|
| LY400170 | Transient overexpression lysate of cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) (as WB positive control) | 100 ug |
$436.00
|
|
| TP305760 | Recombinant protein of human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), 20 µg | 20 ug |
$737.00
|
|
| TP750202 | Purified recombinant protein of Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), full-length, with N-terminal Myc-ddk tag, expressed in E.coli, 50ug | 50 ug |
$260.00
|
|
Citations
Development and Applications of a Zebrafish (Danio rerio) CYP1A-Targeted Monoclonal Antibody (CRC4) with Reactivity across Vertebrate Taxa: Evidence for a Conserved CYP1A Epitope
Anderson, Dubanksy, Wilson et al
Toxics (2022) 10 (7) DOI: 10.3390/toxics10070404
Anderson, Dubanksy, Wilson et al
Toxics (2022) 10 (7) DOI: 10.3390/toxics10070404
Cited by 5
AI Summary: OriGene’s recombinant human CYP1A1 HEK cell lysates were used as positive controls in immunoblotting experiments to validate the cross-species reactivity of the newly developed zebrafish CYP1A-targeted monoclonal antibody (CRC4).
Snippet: S-PAGE-immunoblotting by boiling for 7 min in reducing sample buffer (Thermo Fisher J61337. AD) containing 50 µM PMSF. Commercially sourced HEK cell lysates over-expressing recombinant human CYP1A1 (Origene, Rockville, MD, USA) were prepared similarly. ZFL, PLHC-1, and HEPA1C1C7 cells were grown to near confluence in 6-well plates (Primavera, Corning). Plates seeded with ZFL cells and PLHC-1 cel
CNX-013-B2, a unique pan tissue acting rexinoid, modulates several nuclear receptors and controls multiple risk factors of the metabolic syndrome without risk of hypertriglyceridemia, hepatomegaly and body weight gain in animal models
Sadasivuni, Reddy, Singh et al
Diabetol Metab Syndr (2014) 6 (1), 83 DOI: 10.1186/1758-5996-6-83
Sadasivuni, Reddy, Singh et al
Diabetol Metab Syndr (2014) 6 (1), 83 DOI: 10.1186/1758-5996-6-83
Cited by 11
AI Summary: OriGene's hRXR α, β, and γ over-expressing vectors were used in this study to assess the activation potency (EC50) of CNX-013-B2 on different RXR isoforms, facilitating evaluation of the compound’s selectivity and mechanism of action.
Snippet: For assessing EC50 of activation of RXR isoforms 2 μg of hRXR α, β, or γ (OriGENE, USA) over-expressing vector, 1 μg of plasmid expressing firefly luciferase under RARE element (RARE-Luc) and 25 ng of renilla luciferase vector (QIAGEN, USA) were co-transfected ... Cite SaveSaving
Showing 1-2 of 2 papers.
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