CYP1A1 (NM_000499) Human Tagged ORF Clone
SKU
RC205760
CYP1A1 (Myc-DDK-tagged)-Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1)
$715.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CYP1A1 |
| Synonyms | AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000499 |
| ORF Size | 1536 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000499.5 |
| RefSeq Size | 2608 bp |
| RefSeq ORF | 1539 bp |
| Locus ID | 1543 |
| UniProt ID | P04798, A0N0X8 |
| Cytogenetics | 15q24.1 |
| Domains | p450 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tryptophan metabolism |
| MW | 58.2 kDa |
| Summary | This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jan 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000499" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205760L1 | Lenti ORF clone of Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), Myc-DDK-tagged | 10 ug |
$1,015.00
|
|
| RC205760L2 | Lenti ORF clone of Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), mGFP tagged | 10 ug |
$1,015.00
|
|
| RC205760L3 | Lenti ORF clone of Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), Myc-DDK-tagged | 10 ug |
$1,015.00
|
|
| RC205760L4 | Lenti ORF clone of Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1), mGFP tagged | 10 ug |
$1,015.00
|
|
| RG205760 | CYP1A1 (tGFP-tagged) - Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) | 10 ug |
$915.00
|
|
| SC119858 | CYP1A1 (untagged)-Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) | 10 ug |
$716.00
|
|
| SC322548 | CYP1A1 (untagged)-Human cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) | 10 ug |
$716.00
|
|
Citations
Similarities in mRNA expression of peripheral blood drug metabolizing enzymes and cancer marker genes with biopsy samples of head and neck cancer patients
Hasan, Katiyar, Maurya et al
Biomarkers (2019)
Hasan, Katiyar, Maurya et al
Biomarkers (2019)
AI Summary: OriGene Technologies' gene-specific qPCR template standards for CYP1A1, CYP1B1, CYP2E1, and CYP2A6 were used to generate standard curves, enabling accurate quantification of mRNA expression levels in this study.
Snippet: hydrocarbons (PAHs) such as CYP1A1, 1B1, 2E1 and 2A6, the number of copies of mRNA was calculated by interpolation from the standard curve generated using the known amount of gene-specific qPCR template standards procured from Origene Technologies
Structural and Functional Assessment of Perilipin 2 Lipid Binding Domains(s)
Najt, Lwande, McIntosh et al
Biochemistry (2014)
Najt, Lwande, McIntosh et al
Biochemistry (2014)
Cited by 61
AI Summary: OriGene’s pQE9-His vector was used to clone the full coding sequence of mouse Plin2 cDNA, enabling the expression of recombinant Plin2 and its mutants for structural and functional analyses in this study.
Snippet: Plasmids. The full coding sequence of mouse Plin2 cDNA was cloned into pQE9-His vector (Origene, Rockville, MD) following standard procedures. Unique restriction sites BamHI and HindIII were introduced by PCR amplification with the following forward and reverse ... Cite SaveSaving
Trastuzumab-induced recruitment of Csk-homologous kinase (CHK) to ErbB2 receptor is associated with ErbB2-Y1248 phosphorylation and ErbB2 degradation to mediate cell growth inhibition
Dokmanovic, Wu, Shen et al
Cancer Biol Ther (2014) 15 (8), 1029-41 DOI: 10.4161/cbt.29171
Dokmanovic, Wu, Shen et al
Cancer Biol Ther (2014) 15 (8), 1029-41 DOI: 10.4161/cbt.29171
Cited by 55
AI Summary: OriGene products, specifically the CHK (Csk-homologous kinase) expression plasmid, were used to overexpress CHK in breast cancer cells to investigate its role in mediating ErbB2-Y1248 phosphorylation, Akt downregulation, and cell growth inhibition in the context of trastuzumab treatment.
Showing 1-3 of 3 papers.
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