CDK4 (NM_000075) Human Over-expression Lysate
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC201156 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_000075, NP_000066 |
| Synonyms | CMM3; PSK-J3 |
| Predicted MW | 33.7 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_000066 |
| Locus ID | 1019 |
| Cytogenetics | 12q14.1 |
| Protein Families | WB |
| Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer, T cell receptor signaling pathway, Tight junction |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CDK4" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH301156 | CDK4 MS Standard C13 and N15-labeled recombinant protein (NP_000066) | 10 ug |
$3,360.00
|
|
| LY400021 | Transient overexpression lysate of cyclin-dependent kinase 4 (CDK4) (as WB positive control) | 100 ug |
$436.00
|
|
| TP301156 | Recombinant protein of human cyclin-dependent kinase 4 (CDK4), 20 µg | 20 ug |
$737.00
|
|
Citations
Abemaciclib is a potent inhibitor of DYRK1A and HIP kinases involved in transcriptional regulation
Kaltheuner, Anand, Moecking et al
Nat Commun (2021) 12 (1), 6607 DOI: 10.1038/s41467-021-26935-z
Kaltheuner, Anand, Moecking et al
Nat Commun (2021) 12 (1), 6607 DOI: 10.1038/s41467-021-26935-z
Cited by 50
AI Summary: OriGene contributed to this study by providing the plasmid encoding full-length human wild-type Cdk4 (NM_000075), which was used for protein expression and subsequent structural and functional analyses.
Snippet: ut using a preparative HiLoad 16/60 Superdex 75 prep grade column (Cytiva). A plasmid encoding full-length human wild-type Cdk4 (residues 1–303, UniProt accession number P11802) was purchased from Origene (NM_000075), PCR-amplified with BamHI/EcoRI restriction sites, and cloned into a pACEBac1 expression vector modified with an N-terminal His6 affinity tag followed by a TEV protease cleavage si
miR-6883 family miRNAs target CDK4/6 to induce G1 phase cell cycle arrest in colon cancer cells
Lulla, Slifker, Zhou et al
Cancer Res. (2017)
Lulla, Slifker, Zhou et al
Cancer Res. (2017)
AI Summary: OriGene's CDK4-Luciferase construct was used in this study to assess the direct targeting of CDK4 by miR-6883 family miRNAs in colon cancer cells.
Snippet: … siRNA for CDK4 and CDK6 (sc-29261 and sc-29264) were purchased from Santa Cruz Biotechnology. The CDK4-Luciferase construct was obtained from Origene Technologies. …siRNA for CDK4 and CDK6 (sc-29261 and sc-29264) were purchased from Santa Cruz Biotechnology. The CDK4-Luciferase construct was obtained from Origene Technologies. …siRNA for CDK4 and CDK6 (sc-29261 and sc-29264) were purchased from Santa Cruz Biotechnology. The CDK4-Luciferase construct was obtained from Origene Technologies. …
Combined CDK4/6 and mTOR inhibition is synergistic against glioblastoma via multiple mechanisms
Olmez, Brenneman, Xiao et al
Clin. Cancer Res. (2017)
Olmez, Brenneman, Xiao et al
Clin. Cancer Res. (2017)
miRNA-145 inhibits non-small cell lung cancer cell proliferation by targeting c-Myc
Chen, Zeng, Guo et al
J Exp Clin Cancer Res (2010) 29 (1), 151 DOI: 10.1186/1756-9966-29-151
Chen, Zeng, Guo et al
J Exp Clin Cancer Res (2010) 29 (1), 151 DOI: 10.1186/1756-9966-29-151
Cited by 232
AI Summary: In this study, OriGene products—specifically the c-Myc expression plasmid (RC208447) and c-Myc antibody (TA500259)—were used to investigate and validate the role of c-Myc as a direct target of miR-145 in non-small cell lung cancer cell proliferation.
Showing 1-4 of 4 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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