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MC1 Receptor (MC1R) Human Gene Knockout Kit (CRISPR)

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SKU
KN403218
MC1R - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
  $1,657.00
3 Weeks*

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Other products for "MC1 Receptor or NM_002386"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 linear donor
Vector EF1a-GFP-P2A-Puro
Target Symbol MC1 Receptor
Locus ID 4157
Components

KN403218G1, MC1 Receptor gRNA vector 1 in pCas-Guide CRISPR vector

KN403218G2, MC1 Receptor gRNA vector 2 in pCas-Guide CRISPR vector

KN403218D, Linear donor DNA containing LoxP-EF1A-tGFP-P2A-Puro-LoxP:

The sequence below is cassette sequence only. The linear donor DNA also contains proprietary target sequence.

LoxP-EF1A-tGFP-P2A-Puro-LoxP (2739 bp)

ATAACTTCGT ATAATGTATG CTATACGAAG TTATCGTGAG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA CTTCCACCTG GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA AGTGGGTGGG AGAGTTCGAG GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT GAGGCCTGGC CTGGGCGCTG GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT CTCGCTGCTT TCGATAAGTC TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT TTTTTCTGGC AAGATAGTCT TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT TTTGGGGCCG CGGGCGGCGA CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG GGCCTGCGAG CGCGGCCACC GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT CTGGTGCCTG GCCTCGCGCC GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG TCGGCACCAG TTGCGTGAGC GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA AAATGGAGGA CGCGGCGCTC GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG GCCTTTCCGT CCTCAGCCGT CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG CACCTCGATT AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGGA AGCGGAGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGAG ACGTGGAGGA GAACCCTGGA CCTATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAATAAC TTCGTATAAT GTATGCTATA CGAAGTTAT

More linear donor DNA can be ordered separately

Cat# KN403218D
Description 10 ug linear donor DNA for KN403218 kit
Price $515/€515
If you want to order 20ug, please order quantity of 2 for KN403218D
If you want to order 50ug, please order quantity of 5 for KN403218D

Other selection marker is available for donor DNA.
Please contact techsupport@origene.com for a custom quote.

different donor dna img
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_002386
UniProt ID Q01726
Synonyms CMM5; MSH-R; SHEP2
Summary This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. provided by RefSeq, Jul 2008
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Documents
Resources
Other Versions
SKU Description Size Price
GA102834 MC1R CRISPRa kit - CRISPR gene activation of human melanocortin 1 receptor 1 kit
$1,657.00

file-alt Citations

A proopiomelanocortin-derived peptide sequence enhances plasma stability of peptide drugs
Löw, Roulin, Kunz
FEBS Lett (2020) 594 (17), 2840-2866 DOI: 10.1002/1873-3468.13855
Cited by 6
AI Summary: In this paper, OriGene products—specifically the human POMC cDNA clone (RC216541) and the human POMC shRNA plasmid (TL314880)—were used to facilitate the expression and knockdown of POMC in cellular experiments, enabling the study of plasma-stabilizing properties of POMC-derived peptide sequences.
MC4R-dependent suppression of appetite by bone-derived lipocalin 2
Mosialou, Shikhel, Liu et al
Nature (2017) 543 (7645), 385-390 DOI: 10.1038/nature21697
AI Summary: OriGene products contributed to this study by providing expression constructs for MC4R, MC3R, and MC1R, which were used in binding assays to investigate the interaction between lipocalin 2 and melanocortin receptors.
Snippet: or binding assays, a 100-mm dish of HEK293T cells was trans - fected with 10 μ g of the expression construct containing MC4R , MC3R or MC1R (purchased from Origene Technologies) using Lipofectamine 2000 (Invitrogen, Chicago, IL) according to the manufacturer’s instructions
Synthesis and characterization of a melanoma-targeted fluorescence imaging probe by conjugation of a melanocortin 1 receptor (MC1R) specific ligand
Tafreshi, Huang, Moberg et al
Bioconjug Chem (2012) 23 (12), 2451-9 DOI: 10.1021/bc300549s
Cited by 33
AI Summary: OriGene products contributed to this study by providing the human MC1R cDNA (OriGene, Cat# SC116077) used to engineer A375/MC1R cells for in vitro and in vivo evaluation of the melanoma-targeted fluorescence imaging probe.
FigureFigure
Receptor-mediated transcytosis: a mechanism for active extravascular transport of nanoparticles in solid tumors
Lu, Xiong, Zhang et al
J Control Release (2012) 161 (3), 959-66 DOI: 10.1016/j.jconrel.2012.05.014
Cited by 84
AI Summary: OriGene’s GFP-tagged open reading frame clone of Homo sapiens MC1R was used for transient transfection to enable melanoma cells to express MC1R, facilitating the study of receptor-mediated nanoparticle transport in tumors.
Snippet: tumor uptake may be due at least in part to the enhanced permeability and retention (EPR) effect of long-circulating nanoparticles ... of MC1R, transient transfection of plasmid construct encoding GFP-tagged open reading frame clone of Homo sapiens MC1R (Origene) was carried
Showing 1-4 of 4 papers.
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