Tuba1a Mouse Gene Knockout Kit (CRISPR)

CAT#: KN318458

Tuba1a - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Tuba1a
• Locus ID: 22142
• Components:

  KN318458G1, Tuba1a gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCCAGCAGGCATTGCCGATC

  KN318458G2, Tuba1a gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CATCTCCATCCATGTTGGCC

  KN318458D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TTTGTCTAGC TTGTCGACTA GCCAAAGTAC TGATGGGGTC TGTCTGCAGA AGGGTGTGGC TCCTGGCCTC AGCCGCACTG CAGATGTCAG TGTTGAAATA GAAGGTCCTA TAGTGTGAGA CTCTTTATTA TGTAGCCTAG CCAGAGTACT GATGGGGTCC ACAGAAGGGT GTGGCTGTTG GCCCCAGCCG CACTGCAGAT GTCCATGATG AAATACCAGG TCCTATAGGA TGAAAACTTC ATCCTTACTT ATCGGGTAAA GAAGCAATTA GTATTATTAT GGATAATTAT TTTGTTTTAC AGAATCTATG CTTTAAATAT TTTTCAAATA TGAAAGCTGT TCTATTCAAG CAAGGCTTTG GAAACAATGG GTTTGCACCA TGTCTTAAGC AGTATTAAGG TGACATCTAG GCCACACCCA CTTTTGGTGT TTGTAGTGAG TACTCCTCAT TGTTCCTGTT ATTAGGGATG TGACTCCAAA TAAATTTCAA CAATATTTCT ATTTTTAGAA ACACTTTAGA AATCTATTAA GATCTAAAAT AGAAATACAG CTTAATATTA TAACAGCTTA ATTTTATAAT TTTTTTCCCT CTTTCCACAG AACATGGCAT CCAGCCTGAT GGCCAGATGC CAAGTGACAA GACCATTGGG GGAGGAGATG ACTCCTTCAA CACCTTCTTC AGTGAGACAG GAGCTGGCAA GCATGTGCCC CGGGCAGTGT TCGTAGACCT GGAACCCACG GTCATCGGTA AGCTGGCTGG GGGCTTCCCC AGGCATTCCT TCTTGGTCAC ATACTCAGAA TCTCTCTCCA GACCCAGAAG GGTGAGTGAG CTTGTGTGTG TCCTCGGTGG CCGATGGCTG ACGTCTTCTC CCTTTGCAGA TGAAGTTCGC ACCGGCACCT ACCGCCAGCT CTTCCACCCT GAGCAGCTCA TCACAGGCAA GGAGGATGCT GCCAATAACT ATGCTCGTGG CCACTACACC ATTGGCAAGG AGATCATTGA CCTTGTCCTG GACAGGATTC GCAAGCTGGT AAATGCCATA TATGGATGGG TTTAAAATGC AGTGGGAGGG TGTCTTGGTA TCTGTCAGAA TCTGTCAAGT CTTTTCTATC AAGCAAATAT GGCTCCCAAT CTAAAGGAAT TGCAAATAGT GCTAAGAGTA TGCCCTCCTA ATAAAACATT ACATTTCCTT TTCTCCCACA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_011653
• UniProt ID: P68369
• Synonyms: Tuba-1; Tuba1
• Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[UniProtKB/Swiss-Prot Function]