Traf3ip2 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN318125RB

Traf3ip2 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: Traf3ip2
• Locus ID: 103213
• Components:

  KN318125G1, Traf3ip2 gRNA vector 1 in pCas-Guide CRISPR vector

  KN318125G2, Traf3ip2 gRNA vector 2 in pCas-Guide CRISPR vector

  KN318125RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_134000
• UniProt ID: Q8N7N6
• Synonyms: Act1; AI429613; CIKS
• Summary: E3 ubiquitin ligase that catalyzes 'Lys63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (By similarity). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (By similarity). Essential adapter molecule in IL17A-mediated signaling (PubMed:19825828). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed:19825828).[UniProtKB/Swiss-Prot Function]