Mfn1 Mouse Gene Knockout Kit (CRISPR)

SKU KN310033BN

Mfn1 - mouse gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Vector: mBFP-Neo
• Target Symbol: Mfn1
• Locus ID: 67414
• Components:

  KN310033G1, Mfn1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN310033G2, Mfn1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN310033BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_024200
• UniProt ID: Q811U4
• Synonyms: 2310002F04Rik; 6330416C07Rik; D3Ertd265e; HR2; mKIAA4032
• Summary: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:24513856, PubMed:15297672). Membrane clustering requires GTPase activity (By similarity). It may involve a major rearrangement of the coiled coil domains (PubMed:15297672). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12527753). Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity (By similarity).[UniProtKB/Swiss-Prot Function]
• Shipping: Ambient