Mettl4 Mouse Gene Knockout Kit (CRISPR)

SKU KN310008

Mettl4 - mouse gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Vector: GFP-puro
• Target Symbol: Mettl4
• Locus ID: 76781
• Components:

  KN310008G1, Mettl4 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGATCCAGTAACCACCCAGG

  KN310008G2, Mettl4 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGCATCATTTGCCTCCTGGG

  KN310008D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  TCCTATTGCT CTACAACTGA TAACGATTAG GTAGGGCAGG GATCTGAGTA ATGGAGTGAC TTCTGTGGCA ATAGTAGTTT GAAAACACAA CAGCTCTTTT TCCGTTACTT TCTGTGGCCC TCTCTAAATA GATTTATATC ATTTAAAAAA GGGTAAATTC TTATAGGTAA TTACTATCCT TTGACTTGAC AGGACACTCA CCTTTTAAGG ATTTAGTGAC TTGAATACTT GGACCATGTG GACTCGTGCC TTGATTCGAG GACCAAAACA GGACCACAGA GGACACCCGA AGAAAGGAGA ATCAAACTAA GAAGCGTCTT TGACTACATT GGCTGAAAAT TTTGAATGAC CAATTCTGAA ATTAAAAGAC ACTTAAAGTA TCGCATTTTT CCTTAGAGGT GATATGTACA CACTCACCTT GTATGTTTAA ATATAAAACC TTCTTGTGTG TACAAAGTAC ATCGTTGATA TACAGGACAT TAAGAACTTA ATTACAACAT TGGTATCAAC CCAGGACCCC CATACTGAGC TATACTCCCA GTCCTAGTTC TGATTCTTGA TTATCAGTTT TTTGTTTTTG TTTTTTGTTT TTTTGTGAGA AACTTTGCCA GCATCAAGAG TCTTTTTGTA GTAAAAACAA CCCTACTTCT TCTGTCTACA TGGATTCTCT TCAGCTGGAT CCCGGGTCTC CTTTTGGAGC TCCTGCTATG TGCTTTGCTC CTGACTTCAC TACTGTGTCG GGCAATGACG ATGAAGGAAG TTGTGAAGTG ATCACCGAAA AATATGTTTT CCGATCTGAG CTATTTAATG TCACCAAACC TTACATAGTT CCAGCTGTTC ACAAAGAGCG CCAACAAAGT AACAAAAACG AAAATCTAGT GACTGACTAT AAACAAGAGG TCTCTGTTTC TGTTGGGAAG GTAGGAAGTT TTAATTGTCT TTTTTTTTTT TTAACATGAA ACAAAAAATC TCATTAAGTA TCTCAAACAT CTTCCCATTT TCTTTTTTTT TAAAGGTTCT AAGATTTTTT TATTTTTTTA TAGTTTTTTT TTCCAATTTT TTTATTAGGT ATTTACTTCC TTTACATTTC AAATGCTATC CCGAAAGTTC CCTATGCCCC CCTACCCCCA CCTTCCCATT TTCTAAGTAT AAAAAAGAGT TAATAATGCA GACGGTGTTA GCATGTCCAA GAGCAGTTGT

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_176917, NM_001357135, NM_001357136, NM_001357137, NM_001357138
• UniProt ID: Q3U034
• Synonyms: 2410198H06Rik; A730091E08Rik; AV296509; HsT661
• Summary: Probable methyltransferase.[UniProtKB/Swiss-Prot Function]
• Shipping: Ambient