Met Mouse Gene Knockout Kit (CRISPR)

CAT#: KN309983RB

Met - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: Met
• Locus ID: 17295
• Components:

  KN309983G1, Met gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTTGGTGCAGAGGAGCCATG

  KN309983G2, Met gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTTCATCTCAGACTTCACTA

  KN309983RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  Homologous arm and RFP-BSD sequences:

  pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

  ACACACACAC ACAAATCCTA TAAACAGCGT GTGATTGCTT GCTTCTAGGA TTTATGATTG TTTTCTTCCA AAAGGAAGCT AAATTTAGCC TAGTGATTCT ACATGCTCCC AAGGAAACAA TGCCTGCTGT GATTTCTAGA ATAAATGAAT CTGAGCCACA GTTCCTTTAC TTGACTAAAC AAGAAAGTTT AAGCATCAAC CTAGTCATTA GCCACAGATA GAAACCCACA TTAAGCAAGC AGCAAGGGTT AAGACACAAT GTGCTGTGTT TCTTATTGAG CTGCTGAATG CCATTCAGTT GCTGTGTGGC CTCAACACAG AACATTGTTT GCATCACAGA ATGGTCCTTG AACATGACTC GTTTGTGGCC TGACTTCTAA AATTAAACTA TGTGGAGTCA TGTCCAGCCC TACAATACAT CTTTATGTGA AGCTAGCTAG CAGTTTTTTT CTTTTTCATT CTATGTAAAT AAAATTCCAG TTAGGAAATG TTATTTCTGA TATCGAAGCT GGAGGAGTCA TGCTTCCATT TCTCTCTTTG TTTCTAATGA CGACTAAAGG GCTCTTGCCT GAATGTGTCT TGGCAGATCA ACTCCTCACA CAGCTCCCCA ACTTCACGGC AGAAACCCCC ATCCAGAATG TCGTCCTACA CGGCCATCAT ATTTATCTCG GAGCCACAAA CTACATTTAT GTTTTAAATG ACAAAGACCT TCAGAAGGTA TCCGAATTCA AGACCGGGCC CGTGTTGGAA CACCCAGATT GTTTACCTTG TCGGGACTGC AGCAGCAAAG CCAATTCATC AGGAGGGGTT TGGAAAGACA ACATCAACAT GGCTCTGCTT GTTGACACAT ACTATGATGA TCAACTCATT AGCTGTGGCA GTGTCAACAG AGGGACTTGC CAGCGGCATG TCCTTCCTCC TGACAATTCT GCTGACATCC AGTCTGAGGT CCACTGCATG TTCTCCCCAG AAGAGGAGTC AGGGCAGTGT CCTGACTGTG TAGTGAGTGC CCTCGGAGCC AAAGTCCTCC TGTCGGAAAA GGACCGGTTC ATCAATTTCT TTGTGGGGAA TACGATCAAT TCCTCCTATC CTCCTGGTTA TTCACTGCAT TCGATATCGG TGAGACGGCT GAAGGAAACC CAAGATGGTT TTAAGTTTTT GACAGACCAG TCCTATATTG ATGTCTTACC AGAATTCCAA

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_008591
• Synonyms: AI838057; c-Met; HGF; HGFR; Par4