Lgals1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN309240

Lgals1 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Lgals1
• Locus ID: 16852
• Components:

  KN309240G1, Lgals1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGAGGGTCCCGCACTCACAC

  KN309240G2, Lgals1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GACAATTGGGGACTTGCTTC

  KN309240D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  GGTGGGGCCT GTGCTTTGTG GTCCTCCTCA GTCCTCCCGC AGAAGCCCAC AGTGGGAGTC ACAGCGAGAG CCACACCCTG GCCTGCCAGG CGGCAGCCTC TTCAGAGAGC CTTGCTCTAA TTCTCTGCAG GCATCTATAC ACACATCCCC CTCCCCCACC AGCTGCGCTG GGACCTAGGG GCTTTCTCCC TGCTGGCCCC AGGCCCTATC CCGCCAACTC TTAGGTTTAA CTCATCCTTA AACTATTTTC CATTGGCTCC CCCAATCTCT GCCACAGCAC AAATCTTTTT TTAGTGGAAA GAGGGATGGA GAAGAATAAG AGGCGGCGCC AGGACCCTGA GGGAGGGGCT AGGAGGGGGG GTCTCCTCTC TGACTGGTCA CCTCTGCTCC AAAATTGACT TTATAATCCA TAGACTCCAC TTCTGGTGGC CCCCATCCTT GTCCTGACAT GCAATTGGCT GAACTCCCGG GGAGGGGCGG GACTCACCGG GTCTGATCCA GTTAAAAAGG TCGGAGCGGG CCTGGGGCCC GTCTCTCGGG TGGAGTCTTC TGACTGCTGG TGGAGCAGGT CTCAGGAATC TCTTCGCTTC AGCTTCAATC TGCCTTGGAC AGGTGTTTTC TCTGGTCCTC AGTGGCTACA TCTGTAAAAT GGGGGTGGCT GTCACTGTTC CCCCCTGAAA CCTTAAGCGT CTCTGGCTTA AGAATGTTCT GGACTTCTCG TAGTTCTGGC CGCATACCTG CCTTGCAAAG TCCAGTATTC TGCTGCTTTG CCGTTTTCTG AGAAGTGGGA GTGGGAAGGG TGTGGCCACC CGGGGAAAAG GGAGGCAGGG GGACATTTTG GCCAAGGCCA TTCCCTGTTC ACTCTTCCTC TCCACTCTAG AGTCTCCTGA CCCCTCCCCC GCTGCAGCTG TCCCCAGTCA CAGGGCCAGG GCTCAGTTGG TCACCCTCTG CTCCAGAAAA CTAAAAAAAA AAAAAAAAAA ACCCTCCCGG GCCTAAGATA GGCTCCAGGC TGGTCAGTGG AGGCGGGTCA GGGCTGGGCG GAGGAAACTG CATGCATTTG CACAGACAGG CAAGCCCTCA GGTATAGGGT TCAAATGTCA AAGAAACCCC TTGCCCAAAT GAACTCCTGT CAACTCACCC AGGTAGGTGC ACAGCAGTGC CCTGATATGT ACTGGGCTCT CTAGCGTGCT AAGTCCACAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_008495
• UniProt ID: P16045
• Synonyms: AA410090; Gal-1; Galbp; galectin-1; L-14.5; L14; Lect14
• Summary: Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.[UniProtKB/Swiss-Prot Function]