Keap1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN308748BN

Keap1 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: Keap1
• Locus ID: 50868
• Components:

  KN308748G1, Keap1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN308748G2, Keap1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN308748BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  Homologous arm and mBFP-Neo sequences:

  pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet

  TGTCTTCTCG CTGTGTCTCT GGAGCTCAGA GCAGTCCCAA ATACATAAAT GGCAGGCTCT GTAAATGTTG GTGTGGTGTT GAAAGGAATC TGACATGTTG GACGAAGGCA AGGGGAGGGA AGGATGGCTG GAACAGTGAA GAGGTTGGAA AGCGGGTGTG GAGTTTTACA GGCCATTGAC GATTTGGGGT TTCCATTCTT GGGCTCGGTG AAAGGTGTTG GGTGATTCTG AGCAGGAAAA GGAACATGAT ATGCCCTGAA GGCCCGCGAG TTGAGAAGTT AGTTTGAATG GAGCCGGCTG TGTCCAGTTT ACTTGGCTTG GCAAAATCTG CACTTAGATA TCATTGCTTA GTCTTGCAAA AAAAGAAGCC TGGCTGGACA TGGTGGCACA TACTGTAATC CCAGCACTCG GGAGGAGCCA GCTTTGGTTG CATAGTGAGT TGGAAGCCAG CCTAGGCTAT GTAAGACCCT GTCTCAAATA AAATAAAATA AAGTGGCAGG GTCTGGTCTA ACCCAGCCTC TGTTCCCAGC GCTGTGCTCT TCCCTCCCTC CAGGCCCCTA ACGGCTAGCA GAGGAACTGT GTCTTGTCAT CAGGAACCCC TATGCCTCCA CGGAGTGCAA GGCAGAGGTG ACGCCCTCGC AGGACGGTAA CCGAACCTTC AGCTACACAC TAGAGGATCA CACCAAGCAG GCTTTTGGCG TCATGAACGA GCTTCGCCTG AGCCAGCAAC TCTGTGACGT GACCCTGCAG GTCAAATATG AGGACATCCC AGCTGCCCAA TTCATGGCTC ACAAAGTGGT GCTGGCCTCC TCCAGCCCAG TCTTTAAAGC CATGTTCACC AACGGGCTTC GGGAGCAGGG CATGGAGGTG GTGTCCATCG AAGGCATCCA CCCTAAGGTC ATGGAAAGGC TTATTGAGTT CGCCTACACG GCCTCCATCT CCGTGGGCGA GAAGTGTGTC CTGCACGTGA TGAACGGGGC GGTCATGTAC CAGATTGACA GCGTGGTTCG AGCCTGCAGC GACTTCCTCG TGCAGCAGCT GGACCCCAGC AACGCCATTG GCATCGCCAA CTTCGCGGAG CAGATCGGCT GCACTGAACT GCACCAGCGT GCCCGGGAGT ATATCTACAT GCACTTCGGG GAGGTGAGTG AAGCCGGGCT TTGGGCTCAG AAGTGGGATA GGCCAGCGTG GCGTTCTCCC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001110305, NM_001110306, NM_001110307, NM_016679
• UniProt ID: Q9Z2X8
• Synonyms: INRF2; mKIAA0132
• Summary: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome (By similarity).[UniProtKB/Swiss-Prot Function]