Irf5 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN308427

Irf5 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Irf5
• Locus ID: 27056
• Components:

  KN308427G1, Irf5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAATGAGAGAGTGTCTTCCC

  KN308427G2, Irf5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AAAACTCAGCAGACTTACTT

  KN308427D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  ATTTAATTTA TCATCATCAG GCTGAGACAG AAAGAAAGCT GCAGTGTTCC TTGGCTCCTT CTTGGTCTCC GAAGCAGGGC AGTGAGTGCT CTGCTCTTCC TGTTCATACT ACTCTTGACA TGACTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TACATCACCC TCCATTCTAA GATCAGAGCT GCTTAGGAGC TATAATGGGA GACAGAGAGG ATGATGTTGA GGATGGGGTG GGGGTGGGTC AGCCGAGTGA GTCAGATCTC TGAGTTGTCC CATCGGGCTA CTTTCATTTC CCTTAGGTCA GGGTGGAGGC TGGCGCAGAA AGAGGAACTA AGCCACCTCT TCTTAAGCTT GGGGGCAGGG CTGGGAGCCT TTCCACGCTC TCTGTCCAAA GCTGGCAGTG CTATTCCTGG GTCTCTGGCC GCTGAGGTAT GCACTGCTCT GAAGTGCTCT GAAGTCTGTA GAGGGTCGCT CCGCTAGCCT GGATGTGGCA TGTAGTAGCC ACAACCCAGA AGACACTAGG TGGGGGAATT GCAGGGGAAA CTGGGGCTCC CGCAGCCTTC CTCTTCTCTG TTTCTGAACC CCGCCCCACC CCCACCCCAA GTTCAAGAGT TCAAGATCAA GAATATGGTC AAGGGCAGGA AAGAGCTTCC CAGGAGGGAA GATGGGTGAG AGGGAGGGAG CTCTAAGTTA CAGGTTCTGT CAGGAGGAGA GGGAGCAGGT AGAGGGAAGA GCACCAGGGT TTCATGGGTC CTGTGCACAT AACTCTAGTG AGATAGAGAC TGTGGCCTGC CAAAGTGCTT ACCTCCAATG GAAAGCTGTT GTGGCAATAT TTAATCTCAA TGGGGGGGGG GTGGACTACC TTGCCTTTGA TCATTCAGTT CCCAGATAAA AGGCACTCAG CTTTTATATT TATAACGATC CTTTAGTGTA CCAGAGCCGG GCACATATCA CCCTTCATGC TCTAATGTCT TCTTCCCCAT CAAGAACCCC TCCATGTTCC AACTTAGCTG CTCTTAATTC CAATCGGCCA GCCCTCGTGG CCATGCTTTC ATGAGGCGTT TTCTCCTCCC TCTACCTTGT TGTCTCCCCT CAGACCAAGG AACTCTGCCT ATCTCTCTTC TGCCCAGCTT CAGGCTGTTG GCATCTTTAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001252382, NM_001311083, NM_012057
• UniProt ID: P56477
• Synonyms: AW491843; mirf5
• Summary: Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling (By similarity).[UniProtKB/Swiss-Prot Function]