Gnrh1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN307104RB

Gnrh1 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: Gnrh1
• Locus ID: 14714
• Components:

  KN307104G1, Gnrh1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN307104G2, Gnrh1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN307104RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_008145, NR_133010
• UniProt ID: P13562
• Synonyms: Gnrh; Gnrh2; hpg; LHRH; Lhrh1; Lnrh
• Summary: This gene encodes hypophysiotropic peptides belonging to the family of gonadotropin-releasing hormones that stimulate the release of gonadotropins and suppress secretion of prolactin from the pituitary gland. The encoded protein is proteolytically processed to generate two biologically active mature peptides. A deletional mutation encompassing the distal half of this gene in mice resulting in the loss of the encoded protein leads to hypogonadism and infertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]