Gfpt1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN306419

Gfpt1 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Gfpt1
• Locus ID: 14583
• Components:

  KN306419G1, Gfpt1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CGTGTCAACAGAGCGGCCTT

  KN306419G2, Gfpt1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CTTGTGGAGCGGGCATCCCG

  KN306419D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AGTGAGGAAG GCAGGGAGTG GGGCGCTGAG CACCTTGCCT ACAGCTCAGG TCGTCACCGG AATGGCTGCC CGAGGGCGTC CTGCAGGCTC CCGCACCTCT GCGCCAGCAG CGCAGTTCCG GCCACCGTGG CTCTGCACAC TGACACGTCT GTCCCAGGCT CTCCCTCCTG CCCTACAGCT TTTCTCTCTG TCTCCCTCCT TCCCGTTCCC CTTGAAACAC ACAAACTGCT GTGTAAACAT TCCCTTCCTC CTCCTCCTCC GAGGCTCGGC TCGCCCTCAG CGGGCGGCTC CAGGAACCTA AAGCGGATGC TGGTCGAGCC CCTGCCACGT CGCTACCCGC AGCTCTGCGT CTGCGCGCTG GGCGGGCGAT GCCTGCGCGT GAGCGGCGCG GGCGCGGCCC AATGGGAGAG CCGCGGCGGC GCGCGTGGCC GGGGGGGGCG GGGCGGCAGT TGAGAGCGAA GCGAGCGCTG AGTCGGACTG TCGGGTCTGA GCTGTCGCAT CCCAGAGTCC TCTCATTGCC ACCACCCCGG CCCGAGCTCA CCCTCGCTTC TGAAGCTCTC CGCGCGCCCG ACAGCTCAGC CCTCGCCCGT GACCAACATC GAAAGGCTGC TGTCCGTGAG GCAGGGACTC TAAGATGGGG ATTCCGGTGT TGAAGGGGCG GCCGGAGCTA GTGTGCTTAA GAGGGACCGG CAGAGGAGAT TGTGGGGGCC CTAGGAGTCA GAGGCTACTT AAAGGGGCTA GAGGATTGGG GGTGGGGTGG GCCAGGGAGG GTATCAGAGC GTTCAAGGGG CACCTTTGCA TAGAGCTCAG AGGATAGATG AATACCTACC TTAGAAGAGC TGTGAGGCTC AAAGTGAGAC CAAGTATGGA GGGATGCCTA GGGGAAGGTC CACCAGGTGA GTGAGGCGAC CTGTGAACAT GCTGTGTGGT ACTGGGGGTT AAACTCATAA AGGCAGCTTG TCAAGTGAGT GAGTACTCTT TCCCTTAGCC ACACCCCCCA AATCCCCTTT TTCTGTCTCT TTGTAACTGG GTCCCACTTG GTAGCCCAGG TTGGCCTGTA ATTCACATTG CCCTGTCTCA GCCTTCCAAG TGTTGTGATT ATAAGTATGA ATGACCACTC CTGGCTGGTA GCATTTCTTG AATTTACTTA TCTTGCAGTC TTAAAAATAC TTAATTCTAG TCACTTTATT TTAGGCAGGC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_013528
• UniProt ID: P47856
• Synonyms: 2810423A18Rik; AI324119; AI449986; GFA; GFAT; GFAT1; GFAT1m; Gfpt
• Summary: Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.[UniProtKB/Swiss-Prot Function]