Eef2k Mouse Gene Knockout Kit (CRISPR)

CAT#: KN305017LP

Eef2k - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: Eef2k
• Locus ID: 13631
• Components:

  KN305017G1, Eef2k gRNA vector 1 in pCas-Guide CRISPR vector

  KN305017G2, Eef2k gRNA vector 2 in pCas-Guide CRISPR vector

  KN305017LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001251826, NM_001267710, NM_001267711, NM_007908
• UniProt ID: O08796
• Synonyms: C86191; eEF-2K
• Summary: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.[UniProtKB/Swiss-Prot Function]