Dag1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN304265RB

Dag1 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: Dag1
• Locus ID: 13138
• Components:

  KN304265G1, Dag1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN304265G2, Dag1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN304265RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001276481, NM_001276482, NM_001276485, NM_001276486, NM_001276492, NM_001276493, NM_001276494, NM_010017
• UniProt ID: Q62165
• Synonyms: D9Wsu13e; DG; Dp71; Dp427
• Summary: This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. A complete lack of the encoded protein in mice results in embryonic lethality due to the disorganization of Reichert's membrane. Chimeric mice deficient in the encoded protein overcome embryonic lethality but develop a progressive muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]