Cdk5rap2 Mouse Gene Knockout Kit (CRISPR)
$1,657.00
4 Weeks*
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
|---|---|
| Vector | RFP-BSD |
| Target Symbol | Cdk5rap2 |
| Locus ID | 214444 |
| Components |
KN303046G1, Cdk5rap2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACTCGGGGATGGAAGAGGA KN303046G2, Cdk5rap2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GCCCTGCCTGGGACCCTCAG KN303046RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences: pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACACTAC CGTTTGTAAT AGCGCTCCAC AGGTGGTATT TTTCTAAATC GAAGCAGAAC TCAATTATCT GAGTCTCAAC TTAAGGCAGA GCCTTTTGGG TAGAAAGAGC CTAAGGAGCG ATTTTGGAGT CATGAGTCTG GAACACCAAA CAGATTGATA CTCTGAGCTG TTCCACCTCC CGCTTAAATT AACTGATCCC GGCGGACTTT TACCCTCAAC AAATATGGCG CTCGCCTTGG CTTCGGCCCG AATGCCCTCA CCCACGAAAC ATGGCTACCG CTTCTTTTCT CCTAGTGTTG CACGCTGGAG TGCGCAGGTT CTGCCCTGAG TTAAGATGGC GTCCAGGCAA AGCTCTTCTT GCCCGGTTTC GGGTCGTTGC AGGGCGGCTT CACCGCGCGT GCCTGGGTGA CCCCGCCTTG TTTCCGTAGG AAGAAGCGCC GGGAAAGATG GCGGCGGCTG CGGTTTGAAT TCCAGCGGCG CCGCTTGACT GAGAACAAGA GCTGGGTTGG AGGGGGCGGG ACCTGGGGAA CCGAGCGCGC CGCGACTGCC GGGGAACCAG TGGCGCTGCC GCCGCCGACA CCGGCGCCAC CACTCTGCGT TCACGCCCTG GCTTTGGGAT TCGTGGTACC TGGGTTGGGG TTCCCGTTGA TGGCGACTCA CTCCCTGGAG GCCTTCGGTT TGCTTGGGGA TGGTCTCGGC TGCGCCCCGA GTGTGGTGGG ACCTGGGTGT CCAGCCAGCA CTACATTGGA CTGTGTGTTG TGTGTTCTGG AGTACTTTGA GGACGATGTG TAGGCTAGGC AGGGGGGCTT TTTGAAAGAG TGTGAGGAGA TGGGTCTAAT TAGTAGGGGG TGGCTGGAAC TGGACATCAG AGCACTTAGG TGGTTGATTC TTGTTTGGGG CTTTGCATTC ATTATGGGGA GTGTAGTTGT TATTTTAGAC TTTGTTTATG GGGTCACGGG ACAAAGTTCT GTGGAGCCTT GGCTAGAGAT GGACAAAATT TAATTAAAAA AACGGCCTAT ACCCTGACCA CGTAGGTGAC CTTGGTAAGT TGACTTCCCC ACTGAAAAGT GTACTAACGC CGATGTGCAC TCACCAAAAA CGGGATGAGA ATGAAAAGAA GCAATGGCTA CCCAGCAGTT GTATTGGCTG ACATTCAACC GTGGTGAAAA GGATGATGAC TACCACTATT ACAAGTTGTG GCTTCTCACT CTCGCCGGTT GGACTTTAGA TCAGAAGGGA TCTTGCTGCC GCCCGAAAGA GGAAGGGCTG GAAGAGGAAG GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATCGE100003, scramble sequence in pCas-Guide vector |
| OTI Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_001313762, NM_145990 |
| UniProt ID | Q8K389 |
| Synonyms | 2900018K03Rik; an; mKIAA1633 |
| Summary | Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion (PubMed:20627074). Involved in regulation of mitotic spindle orientation (PubMed:20460369). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Required for the recruitment of AKAP9 to centrosomes (By similarity). Plays a role in neurogenesis (PubMed:20471352). Contrary to higher mammalian orthologs, including human, chimpanzee, bovine and dog, does not interact with EB1/MAPRE1, therefore its function in the regulation of microtubule dynamics is unclear (PubMed:19553473).UniProtKB/Swiss-Prot Function |
| Shipping | Ambient |
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| Product Manuals |
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| SDS |
Resources
Other Versions
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| GA213212 | Cdk5rap2 CRISPRa kit - CRISPR gene activation of mouse CDK5 regulatory subunit associated protein 2 | 1 kit |
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| KN303046 | Cdk5rap2 - mouse gene knockout kit via CRISPR, HDR mediated | 1 kit |
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| KN303046BN | Cdk5rap2 - mouse gene knockout kit via CRISPR, HDR mediated | 1 kit |
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| KN303046LP | Cdk5rap2 - mouse gene knockout kit via CRISPR, HDR mediated | 1 kit |
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| KN503046 | Cdk5rap2 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. | 1 kit |
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Citations
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
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