Atg7 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN301741RB

Atg7 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: Atg7
• Locus ID: 74244
• Components:

  KN301741G1, Atg7 gRNA vector 1 in pCas-Guide CRISPR vector

  KN301741G2, Atg7 gRNA vector 2 in pCas-Guide CRISPR vector

  KN301741RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001253717, NM_001253718, NM_028835
• UniProt ID: Q9D906
• Synonyms: 1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553
• Summary: This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]